| 查看: 477 | 回复: 7 | |||
| 当前主题已经存档。 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
wuhaofei2006
|
[交流]
求助！帮我翻译一下英文期刊呢！
|
||
| The publication by Tacket and colleagues represented results from the first human Phase I clinical trail testing the immunogenicity of a bacterial antigen expressed in transgenic potato. In this study, 14 adult volunteers ingested transgenic potato or control wild-type potato. The transgenic tubers contained 3.7-15.7ug/g of LT-B. At day 28, 7 days after the third dose, a geometric mean titer of 19.1 IgA anti-LT antibody-secreting cells (ASCs) per106 peripheral blood mononuclear cells were detected and a similar pattern of responses was observed for IgG anti-LT ASCs. Serum antibody responses were also detected in 10 out of the 11 volunteers. Eight out of the 11 who are the transgenic potatoes developed neutralization titers of more than 1：100(titer was defined as the highest serum dilution that showed complete neutralization of biological activity of 100 per gram of toxin in the Y-1 adrenal cell assay). This trail served as a proof-of-principle that humans could develop serum and mucosal immune response to antigen delivered in transgenic potato. More recently,Tacket and colleagues reported on the immunogenicity of LT-B delivered to humans in transgenic corn. In these studies, transgenic corn expression 1 mg of LT-B was fed to volunteers in three doses. The corn was formulated as defatted corn germ meal using standard commercial processing techniques. Seven out of the nine volunteers developed increases in serum IgG anti-LT and four of the volunteers also developed stool IgA. |
回复此楼» 猜你喜欢
请教酰胺缩合反应
已经有4人回复
-
一志愿大连理工制药工程专硕086002，本科211，总分342
已经有0人回复
-
药理学论文润色/翻译怎么收费?
已经有63人回复
-
制药工程专业招收调剂，要求过国家线
已经有0人回复
-
五邑大学药物化学生物学团队-李兰清课题组招收调剂学生
已经有0人回复
-
【招聘】清北（济南）生物科技招聘AI制药研发计算人才
已经有15人回复
-
求蛋白推荐，用于实验教学中蛋白药物的表达
已经有0人回复
-
求蛋白推荐，用于实验教学中蛋白药物的表达
已经有0人回复
-
求蛋白推荐，用于实验教学中蛋白药物的表达
已经有2人回复
-
342一志愿大工制药专硕，本科211，求调剂
已经有1人回复
8楼2006-05-31 09:43:13
wuhaofei2006 
银虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 97.6
- 散金: 315
- 红花: 24
- 帖子: 357
- 在线: 79.3小时
- 虫号: 180489
- 注册: 2006-02-09
- 性别: GG
- 专业: 疫苗学
继续
| In another clinical trail, 20human volunteer two or three doses of transgenic potato expressing 215-751 ug of the Norwalk virus capsid protein. Of the 20volunteers, IgA ASCs, four developed serum IgG and six developed specific stool IgA. The levels of serum antibody, however, were not high. |
2楼2006-05-29 11:17:20
wuhaofei2006
银虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 97.6
- 散金: 315
- 红花: 24
- 帖子: 357
- 在线: 79.3小时
- 虫号: 180489
- 注册: 2006-02-09
- 性别: GG
- 专业: 疫苗学
继续
| Worldwide infection with hepatitisB virus is peobably one of the most important causes of persistent viremia. Approximately two billion people are infection with the virus and there are approximately 300 million carriers. Despite the safety and enormous efficacy of the currently licensed recombinant yeast-derived vaccine, infection with hepatitisB continues to be a major global health problem. |
3楼2006-05-29 11:17:44
wuhaofei2006
银虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 97.6
- 散金: 315
- 红花: 24
- 帖子: 357
- 在线: 79.3小时
- 虫号: 180489
- 注册: 2006-02-09
- 性别: GG
- 专业: 疫苗学
继续
| Several groups have expressed the HBsAg in plant tissues. The very first report published the proof-of-concept that plants(tobacco leave) were captable of expressing a viral vaccine antigen. This was followed by the first report by Thanavala and colleagues in 1995 demonstrating the immunogenicity in mice of plant-expressed HBsAg. In these initial studies, tobacco leaf extracts were purified over sucrose gradients and fractions positive for HBsAg by an Auszyme enzyme-linked immunosorbant assay(ELISA) were pooled, concentrated and used toimmunize mice. The antihepatitis B serum response elicited to the tobacco-dederived recombinant(r) HBsAg was found to be qualitatively similar to that obtained by immunizing mice with yeast-derived rHBsAg. Furthermore, this study demonstrated the ability of the tobacco-derived rHBsAg to prime Tcells to respond to in vitro stimulation in an antigen-specific T-cell proliferation assay. This study thus established that the transgenic tobacco-derived rHBsAg retained the immunological properties needed to elicit both HBsAg-specific B and T-cell responses. These studies were then extended to expressing the HBsAg in transgenic potato tubers and utilizing alternative polyadeylation signals and enhancing retention of HBsAg in the endoplasmic reticulum. |
4楼2006-05-29 11:19:01
10