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【求助/交流】请问日立F-4500检测胞内钙离子数据如何分析？急盼回复！
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用F-4500检测胞内游离钙离子，用fura-2做染料，双波长激发，得到数据保存为txt.它们表示的意义是什么？ ROM Version: 4000 05 Instrument Parameters Measurement type: Intracellular cation scan Reagent name: Reagent 1 Number of Wavelengths: 2 EX WL 1: 340 nm EM WL 1: 510 nm EX WL 2: 380 nm EM WL 2: 510 nm Data mode: Fluorescence Scan time: 600 s Cycle time: 1 s Delay: 0 s EX Slit: 2.5 nm EM Slit: 5.0 nm PMT Voltage: 700 V Kinetic Data Spectrum Start(s) End(s) K-Factor Slope (/min) Activity R R2 F1 6.0 594.0 1.0000 2.9967 2.9967 0.8700 0.7569 F2 6.2 594.2 1.0000 -0.8557 -0.8557 0.5368 0.2881 Ratio 6.0 594.0 1.0000 0.1517 0.1517 0.5920 0.3505 Conc 6.0 594.0 1.0000 38.5655 38.5655 0.3515 0.1235 Average Start(s) End(s) Ave F1 Ave F2 Ave Ratio Ave Conc 6.0 594.0 89.316 37.221 2.486 183.059 Processing Performed Mean Smoothed Number of points: 7 Number of times: 1 Savitsky-Golay Smoothed Smoothing order: 3 Number of points: 7 Number of times: 1 Median Smoothed Number of points: 7 Number of times: 1 Calculation Params Formula 2: C = Kd * (R - Rmin)/(Rmax - R) * F2min/F2max Kd: 224.000 F1min: 71.177 F1max: 125.174 F2min: 46.411 F2max: 19.601 Data Points Time(s) F1 F2 Ratio Conc 6.0 76.10 43.48 1.750 24.779 7.0 76.10 43.51 1.749 24.635 8.0 76.10 43.58 1.746 24.284 9.0 75.92 43.62 1.740 23.612 10.0 75.62 43.65 1.732 22.651 11.0 75.62 43.68 1.731 22.526 12.0 75.62 43.72 1.730 22.316 13.0 75.62 43.98 1.719 21.108 14.0 76.37 44.04 1.734 22.855 15.0 76.81 44.04 1.744 24.035 16.0 77.21 44.04 1.753 25.111 17.0 77.21 43.96 1.756 25.494 18.0 77.21 43.71 1.766 26.733 19.0 77.21 43.46 1.776 27.936 20.0 76.80 43.02 1.785 29.010 21.0 75.96 42.58 1.784 28.844 22.0 75.81 42.50 1.784 28.844 23.0 75.81 42.50 1.784 28.844 24.0 75.81 42.50 1.784 28.844 25.0 75.81 42.54 1.782 28.609 26.0 76.08 42.55 1.788 29.332 27.0 76.08 42.55 1.788 29.332 28.0 76.08 42.55 1.788 29.332 29.0 76.08 42.39 1.795 30.184 30.0 76.01 42.06 1.807 31.680 31.0 76.01 41.76 1.820 33.265 32.0 76.01 41.57 1.829 34.320 33.0 76.01 41.53 1.830 34.508 34.0 76.58 41.53 1.844 36.203 35.0 77.21 41.53 1.859 38.103 36.0 77.92 41.61 1.873 39.820 37.0 78.74 41.93 1.878 40.482 38.0 79.00 42.30 1.868 39.212 39.0 79.00 42.59 1.855 37.613 40.0 79.00 42.64 1.853 37.317 41.0 79.00 42.64 1.853 37.317 42.0 78.30 42.64 1.836 35.249 43.0 77.75 42.42 1.833 34.853 44.0 77.40 41.99 1.843 36.162 45.0 77.40 41.58 1.861 38.429 46.0 77.40 41.19 1.879 40.660 47.0 77.40 41.11 1.883 41.112 48.0 77.49 41.11 1.885 41.376 49.0 77.80 41.11 1.892 42.337 50.0 77.80 41.24 1.886 41.589 51.0 77.80 41.34 1.882 41.025 52.0 77.80 41.36 1.881 40.908 53.0 77.41 41.36 1.872 39.714 54.0 76.83 41.36 1.858 37.945 55.0 76.59 41.35 1.852 37.233 56.0 76.17 41.35 1.842 35.966 57.0 76.17 41.35 1.842 35.966 58.0 76.17 41.36 1.842 35.944 59.0 76.17 41.26 1.846 36.475 60.0 76.58 41.24 1.857 37.871 急盼大家帮助！非常感谢！ |
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2楼2010-10-22 10:04:36
