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emilyye

金虫 (初入文坛)

应助: 0 (幼儿园)
金币: 1012.6
帖子: 47
在线: 26小时
虫号: 477607
注册: 2007-12-13
性别: MM
专业: 医学

[交流] 【求助/交流】请问日立F-4500检测胞内钙离子数据如何分析？急盼回复！

用F-4500检测胞内游离钙离子，用fura-2做染料，双波长激发，得到数据保存为txt.它们表示的意义是什么？
ROM Version: 4000 05

Instrument Parameters
Measurement type: Intracellular cation scan
Reagent name: Reagent 1
Number of Wavelengths: 2
EX WL 1: 340 nm
EM WL 1: 510 nm
EX WL 2: 380 nm
EM WL 2: 510 nm
Data mode: Fluorescence
Scan time: 600 s
Cycle time: 1 s
Delay: 0 s
EX Slit: 2.5 nm
EM Slit: 5.0 nm
PMT Voltage: 700 V

Kinetic Data
Spectrum Start(s) End(s) K-Factor Slope (/min) Activity R R2
F1 6.0 594.0 1.0000 2.9967 2.9967 0.8700 0.7569
F2 6.2 594.2 1.0000 -0.8557 -0.8557 0.5368 0.2881
Ratio 6.0 594.0 1.0000 0.1517 0.1517 0.5920 0.3505
Conc 6.0 594.0 1.0000 38.5655 38.5655 0.3515 0.1235

Average
Start(s) End(s) Ave F1 Ave F2 Ave Ratio Ave Conc
6.0 594.0 89.316 37.221 2.486 183.059

Processing Performed
Mean Smoothed
Number of points: 7
Number of times: 1
Savitsky-Golay Smoothed
Smoothing order: 3
Number of points: 7
Number of times: 1
Median Smoothed
Number of points: 7
Number of times: 1

Calculation Params
Formula 2: C = Kd * (R - Rmin)/(Rmax - R) * F2min/F2max
Kd: 224.000
F1min: 71.177
F1max: 125.174
F2min: 46.411
F2max: 19.601

Data Points
Time(s) F1 F2 Ratio Conc
6.0 76.10 43.48 1.750 24.779
7.0 76.10 43.51 1.749 24.635
8.0 76.10 43.58 1.746 24.284
9.0 75.92 43.62 1.740 23.612
10.0 75.62 43.65 1.732 22.651
11.0 75.62 43.68 1.731 22.526
12.0 75.62 43.72 1.730 22.316
13.0 75.62 43.98 1.719 21.108
14.0 76.37 44.04 1.734 22.855
15.0 76.81 44.04 1.744 24.035
16.0 77.21 44.04 1.753 25.111
17.0 77.21 43.96 1.756 25.494
18.0 77.21 43.71 1.766 26.733
19.0 77.21 43.46 1.776 27.936
20.0 76.80 43.02 1.785 29.010
21.0 75.96 42.58 1.784 28.844
22.0 75.81 42.50 1.784 28.844
23.0 75.81 42.50 1.784 28.844
24.0 75.81 42.50 1.784 28.844
25.0 75.81 42.54 1.782 28.609
26.0 76.08 42.55 1.788 29.332
27.0 76.08 42.55 1.788 29.332
28.0 76.08 42.55 1.788 29.332
29.0 76.08 42.39 1.795 30.184
30.0 76.01 42.06 1.807 31.680
31.0 76.01 41.76 1.820 33.265
32.0 76.01 41.57 1.829 34.320
33.0 76.01 41.53 1.830 34.508
34.0 76.58 41.53 1.844 36.203
35.0 77.21 41.53 1.859 38.103
36.0 77.92 41.61 1.873 39.820
37.0 78.74 41.93 1.878 40.482
38.0 79.00 42.30 1.868 39.212
39.0 79.00 42.59 1.855 37.613
40.0 79.00 42.64 1.853 37.317
41.0 79.00 42.64 1.853 37.317
42.0 78.30 42.64 1.836 35.249
43.0 77.75 42.42 1.833 34.853
44.0 77.40 41.99 1.843 36.162
45.0 77.40 41.58 1.861 38.429
46.0 77.40 41.19 1.879 40.660
47.0 77.40 41.11 1.883 41.112
48.0 77.49 41.11 1.885 41.376
49.0 77.80 41.11 1.892 42.337
50.0 77.80 41.24 1.886 41.589
51.0 77.80 41.34 1.882 41.025
52.0 77.80 41.36 1.881 40.908
53.0 77.41 41.36 1.872 39.714
54.0 76.83 41.36 1.858 37.945
55.0 76.59 41.35 1.852 37.233
56.0 76.17 41.35 1.842 35.966
57.0 76.17 41.35 1.842 35.966
58.0 76.17 41.36 1.842 35.944
59.0 76.17 41.26 1.846 36.475
60.0 76.58 41.24 1.857 37.871

急盼大家帮助！非常感谢！

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