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【求助/交流】着急，Trimmer Direct cDNA Normalization Kit有人用过吗？ 已有1人参与
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有人用过吗……有关于这个试剂盒的详细介绍吗？有人知道这个盒子的基本原理吗?里面那个DSN是什么啊？ [ Last edited by 我和我自己 on 2010-10-9 at 12:53 ] |
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小木虫(金币+0.5):给个红包，谢谢回帖交流
小木虫(金币+0.5):给个红包，谢谢回帖交流
| cDNA normalization is essential for hunting and analyzing the rare transcripts using high-throughput approaches, such as EST sequencing projects, microarrays, functional screenings etc. The Evrogen TRIMMER-DIRECT kit is developed for normalization of cDNA enriched with full-length sequences and intended for directional cloning. While most normalization methods are not well suited for the normalization of long cDNA, TRIMMER-DIRECT allows to obtain normalized cDNA samples in which individual cDNA sizes range from 0.5 to 4.5 kb. The normalization is based on unique properties of novel Duplex Specific Nuclease (Zhulidov et al., Nucl. Acids. Res. 2004, 32: e37). It is performed prior to cDNA cloning, and does not include physical separation steps. Normalization using the TRIMMER-DIRECT kit significantly increases the efficiency of random sequencing of amplified cDNA. |
2楼2010-10-10 11:55:06