| 查看: 391 | 回复: 1 | |||
[交流]
【求助/交流】着急，Trimmer Direct cDNA Normalization Kit有人用过吗？ 已有1人参与
|
|
有人用过吗……有关于这个试剂盒的详细介绍吗？有人知道这个盒子的基本原理吗?里面那个DSN是什么啊？ [ Last edited by 我和我自己 on 2010-10-9 at 12:53 ] |
回复此楼» 猜你喜欢
博士自荐
已经有8人回复
-
298求调剂
已经有3人回复
-
博士推荐
已经有4人回复
-
求环氧树脂研发1名
已经有10人回复
-
280求调剂
已经有5人回复
-
什么是人一生最重要的？
已经有10人回复
-
面上可以超过30页吧？
已经有13人回复
-
为什么中国大学工科教授们水了那么多所谓的顶会顶刊，但还是做不出宇树机器人？
已经有13人回复
-
版面费该交吗
已经有17人回复
-
【博士招生】太原理工大学2026化工博士
已经有8人回复
» 本主题相关商家推荐: (我也要在这里推广)
★
小木虫(金币+0.5):给个红包，谢谢回帖交流
小木虫(金币+0.5):给个红包，谢谢回帖交流
| cDNA normalization is essential for hunting and analyzing the rare transcripts using high-throughput approaches, such as EST sequencing projects, microarrays, functional screenings etc. The Evrogen TRIMMER-DIRECT kit is developed for normalization of cDNA enriched with full-length sequences and intended for directional cloning. While most normalization methods are not well suited for the normalization of long cDNA, TRIMMER-DIRECT allows to obtain normalized cDNA samples in which individual cDNA sizes range from 0.5 to 4.5 kb. The normalization is based on unique properties of novel Duplex Specific Nuclease (Zhulidov et al., Nucl. Acids. Res. 2004, 32: e37). It is performed prior to cDNA cloning, and does not include physical separation steps. Normalization using the TRIMMER-DIRECT kit significantly increases the efficiency of random sequencing of amplified cDNA. |
2楼2010-10-10 11:55:06