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强之寒冬

金虫 (正式写手)

[交流] 【求助/交流】请问MTT和MTS有什么区别! 已有7人参与

以前看文献只看过MTT Assay!  这两天又看到个MTS!  我看具体名称好像都是细胞存活率测试,请问这两种方法有什么区别?
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小木虫(金币+0.5):给个红包,谢谢回帖交流
强之寒冬(金币+2): 2010-10-06 20:09:49
The MTT assay and the MTS assay are colorimetric assays for measuring the activity of enzymes that reduce MTT or close dyes (XTT, MTS, WSTs) to formazan dyes, giving a purple color. A main application allows to assess the viability (cell counting) and the proliferation of cells (cell culture assays). It can also be used to determine cytotoxicity of potential medicinal agents and toxic materials, since those agents would stimulate or inhibit cell viability and growth.
MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole), is reduced to purple formazan in living cells.[1] A solubilization solution (usually either dimethyl sulfoxide, an acidified ethanol solution, or a solution of the detergent sodium dodecyl sulfate in diluted hydrochloric acid) is added to dissolve the insoluble purple formazan product into a colored solution. The absorbance of this colored solution can be quantified by measuring at a certain wavelength (usually between 500 and 600 nm) by a spectrophotometer. The absorption maximum is dependent on the solvent employed.MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium), in the presence of phenazine methosulfate (PMS), produces a formazan product that has an absorbance maximum at 490-500 nm in phosphate-buffered saline.[3](维基百科粘的)
MTS不过是Promega开发的一种新替代实际罢了
7楼2010-10-06 20:04:10
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小木虫(金币+0.5):给个红包,谢谢回帖交流
amisking(金币+1):鼓励下! 2010-10-06 20:01:35
强之寒冬(金币+1):谢谢! 2010-10-06 20:02:05
MTT我以前测酶活的时候用过 具体是什么名字 你说一下 当时测酶活不对头 一看MTS当成MTT了
戒嗔怒以养肝气,省言语以养神气,多读书以养质气,顺时令以养元气,不拘节以养大气,观天变以养灵气,莫强求规于运气。
2楼2010-10-06 18:50:58
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强之寒冬

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amisking(金币+2):鼓励交流! 2010-10-06 20:01:44
MTT法又称MTT比色法,是一种检测细胞存活和生长的方法。(在百度上复制的)
MTS cell viability assays were performed to provide anassessment of the toxicity of the QDs(这是在文献里复制的)  感觉差不多啊!
3楼2010-10-06 19:04:29
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三磷酸腺苷

铁杆木虫 (职业作家)


小木虫(金币+0.5):给个红包,谢谢回帖交流
强之寒冬(金币+2): 2010-10-06 19:42:39
MTS被细胞分解的蓝色产物是可溶的,而MTT的需要加DMSO溶
4楼2010-10-06 19:31:24
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