| 查看: 560 | 回复: 1 | |||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | |||
[交流]
生物学四句翻译
|
|||
| 以牛的抗菌肽（Indolicidin）氨基酸序列为模板，在多肽C端添加Kallikrein蛋白酶切位点设计出新的抗菌肽NK-16检测出抗菌活性。根据氨基酸序列和E.coli偏爱密码子，反向翻译设计并合成四个重叠基因片段，在5’端和3’端分别导入用于表达载体构建的限制性内切位点Xho I和Nco I。利用Overlapping PCR技术，连接合成片段，将连接好的核苷酸序列克隆入pET 30a(+)载体中构建多重载体PET30a(+)-NK-16，经双酶切和PCR鉴定表明多重表达载体PET30a(+)-NK-16构建成功。多重表达载体PET30a(+)-NK-16的正确构建，为抗菌肽的原核高效表达奠定了基础。 |
回复此楼» 猜你喜欢
有时候，自然基金真的不能太认真 （我的申报经验）
已经有11人回复
-
欢迎发来filecode的Mz6后的代码验证其规律
已经有68人回复
-
时间戳又变了8-15
已经有23人回复
-
filecode=后面第一个是大写字母
已经有7人回复
-
filecode
已经有12人回复
-
小木虫上这么多卖论文的，真有人买论文么？感觉没必要啊
已经有13人回复
-
哪位老哥知道今年的国自然具体哪一天放榜？
已经有7人回复
-
关于Filecode分析方法
已经有13人回复
-
各位道友，我要去昆明玩几天，回来见。
已经有8人回复
-
咱们一起用铁证分析2026国家社科基金中标与否
已经有22人回复
ringzhu 
木虫 (职业作家)
- 翻译EPI: 24
- 应助: 5 (幼儿园)
- 贵宾: 1.318
- 金币: 1370.6
- 散金: 8739
- 红花: 60
- 沙发: 11
- 帖子: 4511
- 在线: 703.5小时
- 虫号: 825134
- 注册: 2009-08-10
- 性别: MM
- 专业: 药剂学
fbook(金币+100, 翻译EPI+1): 2010-09-30 18:04:39
|
To detect antimicrobial activity,we take amino acid sequence of Bovine antimicrobial peptide (Indolicidin) as a template, adding Kallikrein protease cleavage sites to the C terminal of peptide to get new antimicrobial peptide NK-16. Based on amino acid sequence and E.coli preferred codons,four overlapping fragments were backward translated ,designed and synthesized. The restriction sites Xho I and Nco I which were used to express the contructino of vector were introduced onto 5 'and 3'terminal. Overlapping PCR technique was used to connect synthetic fragment and made the connected nucleotide sequence cloned into pET 30a (+) vector to construct multi-carrier PET30a (+)-NK-16.the multiple expression vector PET30a (+)-NK-16 was proved to be constructed successfully by the identification by Double digestion and PCR. the right construction of multiple expression vector PET30a (+)-NK-16 lay the groundwork for the highl expression of prokaryotic antimicrobial peptides. |
2楼2010-09-30 11:12:13
