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gaoshandian1金虫 (小有名气)
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表达文库免疫技术（ELI）
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Adel M. Talaat* and Katherine Stemke-Hale Expression library immunization (ELI) is a novel protocol for the systemic screening of any given genome to identify potential vaccine candidates. The basic concept of ELI introduced by Barry et al. is simple yet powerful (9) and can be applied to screen sequenced or unsequenced genomes of infectious agents. In principle, ELI can reduce any pathogen's genome in a relatively unbiased way to only a few antigens that provide protective immune responses. The essential concept in this approach is to employ the host immune system to select the best vaccine candidates against a particular disease. The essence of this approach is that the entire genome of a pathogen (bacterial, viral, parasitic, or fungal) can be cloned into genetic immunization vectors under the control of a eukaryotic promoter to create a library that would express all the open reading frames (ORFs) of a pathogen. Purified plasmid DNA from this library is inoculated into animals, usually in subgenomic pools of clones, to induce immune responses against the cloned antigens. The immunized animals are then challenged with the pathogenic organism to see which clones induced protective immunity and therefore which pool of antigens should be further deconvoluted to individual protective antigens. The main advantage of ELI is twofold; it provides a rapid screening protocol for an entire genome and the readout of screening is often protection, an end goal for vaccine development. In this review, we will focus our discussion on the technical merits of ELI compared to those of other vaccine discovery systems directed against infectious diseases. We will also explain the advantage and limitations of different ELI protocols and their application to search the genomes of bacterial, viral, and eukaryotic parasites. Finally, we will discuss the future of ELI in light of the current genomic revolution. －－－－－－－－－－－－－－－ Infection and Immunity, November 2005, p. 7089-7098, Vol. 73, No. 11 全文链接 |
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2楼2006-05-11 09:28:54