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zjandzq金虫 (正式写手)
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求助翻译一段抗生素抗菌文献
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Thirteen clinical isolates of Escherichia coli resistant to ceftazidime that possessed an AmpC and other (b-lactamases were identified. The effectiveness of different formulations of piperacillin/tazobactam to other b-lactams was compared. Antibiotic susceptibility testing, polymerase chain reaction, amplification of blaTEM, blaSHV and blaAmpC, and enzyme-linked immunosorbent assays to identify AmpC b-lactamases were performed. Hydrolysis rates were obtained and residual enzymatic activity was determined. Cefepime and ertapenem were more active than piperacillin/tazobactam. In contrast, increasing the relative proportion of tazobactam improved susceptibility testing. Twenty micromolar tazobactam inhibited total b-lactamase activity (as measured by nitrocefin hydrolysis rates) by greater than 75% against all isolates tested: in 11 of 13 E. coli isolates, total b-lactamase activity was inhibited by 90%. The observed differences between MIC determinations and susceptibility to enzymatic inactivation by tazobactam against E. coli containing AmpC and other b-lactamases may be due to the final tazobactam concentration achieved in the periplasmic space. Factors determining this are critical considerations in assessing b-lactamase inhibitor potency. |
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anjw_00
铁杆木虫 (知名作家)
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zjandzq(金币+15, 翻译EPI+1):请问Escherichia coli resistant to ceftazidime 翻译为：耐头孢他啶的大肠杆菌么？ 2010-08-12 14:35:38
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十三株临床分离出来的大肠杆菌已经被鉴定出来，它们对可以拥有AmpC（这不太确定）和b-内酰胺酶的头孢他啶具有抗药性。对哌拉西林／三唑巴坦和其他b-内酰胺的 不同配方的有效性进行比较。采用药敏试验、PCR、blaTEM, blaSHV以及blaAmpC的扩增（好像单词拼错了，不知道什么意思）以及ELISA方法对AmpC b-内酰胺酶进行检测。得到水解率和剩余酶的活性。头孢吡肟和厄他培南的活性强于哌拉西林／三唑巴坦。相反，增加三唑巴坦的相对比例可以改善药敏试验。20mol三唑巴坦对所有分离出来的菌株，其抑制总b-内酰胺酶活性（根据nitrocefin的水解率计算）大于75%：在13株大肠杆菌中有11株，总b-内酰胺酶活性被抑制率为90%。三唑巴坦对含有AmpC和其他b-内酰胺酶的大肠杆菌的MIC（最低抑菌浓度？）和酶失活的易感性，二者之间的区别取决于细胞周质间隙的三唑巴坦的最终浓度。决定这些的因素是评估β-内酰胺酶抑制剂效力时至关重要的考虑因素。 |
2楼2010-08-12 14:19:34
anjw_00
铁杆木虫 (知名作家)
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3楼2010-08-12 19:31:28
