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1.实验所选各种蛋白酶最适反应条件 2.DPPH•试验加样表 3.五种酶的酶活力 4.分别经过Protamex、Neutrase、Alcalase、酸性蛋白酶和Papain酶解的5种不同的鲨鱼皮明胶水解产物对DPPH•自由基、ABTS自由基以及对Fe2+诱导卵黄脂蛋白多不饱和脂肪酸的过氧化反应的清除作用 5.明胶水解产物的SP-Sephadex C-25离子交换色谱 6.阳离子交换色谱纯化组分的DPPH•自由基清除活性 7.P2的Sephadex G-50凝胶过滤层析色谱 8.凝胶过滤色谱洗脱组分的DPPH自由基•清除活性 9.明胶水解产物及洗脱峰M2的小分子电泳图 电泳条件：工作电压：浓缩胶，中间胶40V，分离胶60V 10.标准样品电泳迁移率图 |
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yanghong206(金币+10, 翻译EPI+1): 2010-07-31 08:40:10
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1.实验所选各种蛋白酶最适反应条件The optimal reaction conditions of various proteases by experiments 2.DPPH•试验加样表 DPPH•experiment sample table 3.五种酶的酶活力 activities of five enzymes 4.分别经过Protamex、Neutrase、Alcalase、酸性蛋白酶和Papain酶解的5种不同的鲨鱼皮明胶水解产物对DPPH•自由基、ABTS自由基以及对Fe2+诱导卵黄脂蛋白多不饱和脂肪酸的过氧化反应的清除作用 the ability of shark skin gelatin hydrolysis products catalysed by five different enzymes Protamex, Neutrase, Alcalase, acidic protease, and Papain to eliminate DPPH•radicals and ABTS radicals as well we inhibit Fe(II)-induced yolk lipoprotein polyunsaturated fatty acid peroxidation. 5.明胶水解产物的SP-Sephadex C-25离子交换色谱 the SP-Sephadex C-25 cation exchange chromatography of gelatin hydrolysis products 6.阳离子交换色谱纯化组分的DPPH•自由基清除活性 the activity to eliminate DPPH•radicals of components purified by cation exchange chromatography 7.P2的Sephadex G-50凝胶过滤层析色谱 the Sephadex G-50 gel filtration chromatography of P2 8.凝胶过滤色谱洗脱组分的DPPH自由基•清除活性 the activity to eliminate DPPH•radicals of components eluted by gel filtration chromatography 9.明胶水解产物及洗脱峰M2的小分子电泳图 the electrophoresis of hydrolysis products of gelatin and elution peak M2 电泳条件：工作电压：浓缩胶，中间胶40V，分离胶60V electrophoresis conditions: Operating Voltage: 40V for stacking and intermediate gel 60V for separating gel 10.标准样品电泳迁移率图 the electrophoretic mobility plan of standard sample |
2楼2010-07-28 22:11:33
yanghong206(金币+10): 2010-07-31 08:40:16
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有些小改动: 1.实验所选各种蛋白酶最适反应条件The optimal reaction conditions of various proteases by experiments 2.DPPH•试验加样表 DPPH•experiment sample table 3.五种酶的酶活力 activities of five enzymes 4.分别经过Protamex、Neutrase、Alcalase、酸性蛋白酶和Papain酶解的5种不同的鲨鱼皮明胶水解产物对DPPH•自由基、ABTS自由基以及对Fe2+诱导卵黄脂蛋白多不饱和脂肪酸的过氧化反应的清除作用 the ability of shark skin gelatin hydrolysis products catalysed by five different enzymes Protamex, Neutrase, Alcalase, acidic protease, and Papain to eliminate DPPH•radicals and ABTS radicals and inhibit Fe(II)-induced yolk lipoprotein polyunsaturated fatty acid peroxidation. 5.明胶水解产物的SP-Sephadex C-25离子交换色谱 the SP-Sephadex C-25 cation exchange chromatography of gelatin hydrolysis products 6.阳离子交换色谱纯化组分的DPPH•自由基清除活性 the activity to eliminate DPPH•radicals of components purified by cation exchange chromatography 7.P2的Sephadex G-50凝胶过滤层析色谱 the Sephadex G-50 gel filtration chromatography of P2 8.凝胶过滤色谱洗脱组分的DPPH自由基•清除活性 the activity to eliminate DPPH•radicals of components eluted by gel filtration chromatography 9.明胶水解产物及洗脱峰M2的小分子电泳图 the electrophorogram of gelatin hydrolysis products and elution peak M2 电泳条件：工作电压：浓缩胶，中间胶40V，分离胶60V electrophoresis conditions: Operating Voltage: 40V for stacking and intermediate gel 60V for separating gel 10.标准样品电泳迁移率图 the electrophoretic mobility plan of standard sample |
3楼2010-07-28 22:18:03