24小时热门版块排行榜    

Znn3bq.jpeg
查看: 1160  |  回复: 3
本帖产生 1 个 翻译EPI ,点击这里进行查看
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

wd519wd706

银虫 (小有名气)

[交流] 翻译急用,非常感谢你的帮助!!!!

Transcriptomic and Phenotypic Responses of Listeria monocytogenes Strains Possessing Different Growth Efficiencies under Acidic Conditions。In an experiment delineating aciduric strains, food and clinical Listeria monocytogenes isolates tended to produce the most biomass whereas ovine and avian strains produced comparatively less biomass when exposed to high levels of sodium diacetate (SD) and potassium sorbate. Compared to reference strains that exhibited greater acid sensitivity, representative food isolates with comparatively good growth capacities in the presence of 21 mM SD at pH 5.0 accumulated reduced levels of acetate anion and K+ ion. The aciduric nature of SD-resistant strains was also reflected by comparatively high tolerance to pH 2.4 (HCl) acid challenges, a property boosted by the presence of SD. Exposure to elevated levels of SD (21 mM SD at pH 5.0) was found to have broad effects on gene expression, as differentiated from effects caused by mildly acidic conditions (pH 5.0). SD-resistant strain FW04/0025 was more responsive to elevated SD, increasing the expression of 222 genes (>2-fold change [P < 0.05]), compared to the more sensitive EGD reference strain, which exhibited increases in expression of 112 genes. Key differences between the strains in relation to SD-enhanced transcripts were notably associated with the cell envelope, oxidative stress management, and intermediary metabolism. SD thus appears to differentially influence growth efficiency and survival of strains, under conditions relevant to acidic foods, that could be due to altered cell wall and metabolic phenotypes.

» 猜你喜欢

已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

zerohead

禁虫 (职业作家)

引用回帖:
Originally posted by sundaes21 at 2010-07-19 14:34:43:
转录组学以及表象的单核细胞 ...

这可是个攻关题,还是楼上的狠啊。
3楼2010-07-19 14:37:47
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 4 个回答

sundaes21

木虫 (小有名气)

11-247 azider


发芽土豆(金币+1):Thx for your participation! 2010-07-19 16:45:18
wd519wd706(金币+8, 翻译EPI+1):感谢你 2010-07-19 23:26:21
转录组学以及表象的单核细胞增多性李氏杆菌株的反应在酸性的条件下具有不同的生长效率。在实验过程中圈定的嗜酸菌株中,当被暴露在高浓度的双乙酸钠和山梨酸钾条件下时,食品和临床中分离的单核细胞增多性李氏杆菌倾向于产生最多的生物质,而ovine菌株和avain菌株则产生较少的生物质。与对照的菌株相比,以上菌株展现出极强的酸性敏感性,其中生长能力相对好的食品的分离株具有代表性,当它在21mMSD,pH=5.0时,醋酸根离子和钾离子含量降低。这种嗜酸的特性同时也可以通过对于pH=2.4的盐酸的高抗拒性表现出来,这种性能正是引入SD所促进的。在较高的SD存在量下(21mM pH=5.0),与温和的酸性条件下比,其对于基因的表达有着广泛的影响。抗SD的菌株 FW04/0025对于较高的SD含量的响应更为强烈,与更为敏感的EGD对照株相比,其增加了222个基因的表达(),而对照株增加了112个基因的表达。普通的转录与SD加强的转录的最重要的不同点与细胞膜,(氧化应激性),中间代谢有着重要联系。因此对于生长效率以及菌株的存活,在酸性食物的条件下,SD呈现出不同的影响特性,这可能是细胞壁以及代谢表型的变化引起的。
xxxxxxx
2楼2010-07-19 14:34:43
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

sundaes21

木虫 (小有名气)

11-247 azider

引用回帖:
Originally posted by zerohead at 2010-07-19 14:37:47:


这可是个攻关题,还是楼上的狠啊。

犹豫了很久啊。。。呵呵呵
xxxxxxx
4楼2010-07-19 14:44:25
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
普通表情 高级回复 (可上传附件)
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 本科西工大 324求调剂 +3 wysyjs25 2026-04-10 3/150 2026-04-10 18:15 by Kilig0317
[考研] 298求调剂 +8 钉叮咚冬瓜 2026-04-07 10/500 2026-04-10 11:25 by 高维春
[考研] 一志愿中南大学物理学,英一66,求调剂 +4 长烟旖旎 2026-04-08 5/250 2026-04-10 10:31 by 颖果儿
[考研] 化工调剂求导师收留!一志愿失利,踏实肯干,有植物提取科研经历 +17 yzyzx 2026-04-09 17/850 2026-04-10 10:29 by wp06
[考研] 一志愿华中农微生物,288分,三年实验经历 +10 代fish 2026-04-09 10/500 2026-04-10 09:49 by potato妹
[考研] 269电子信息求调剂,可转专业 +9 独酌wl 2026-04-06 9/450 2026-04-09 20:55 by laoshidan
[考研] 计算机类求调剂,22408-274分 +5 上岸de小虫 2026-04-09 6/300 2026-04-09 20:48 by xujun0624
[考研] 一志愿中国科学院上海有机所,有机化学356分找调剂 +11 Nadiums 2026-04-09 11/550 2026-04-09 18:04 by lijunpoly
[考研] 288求调剂 +15 没有答案_ 2026-04-05 15/750 2026-04-09 10:22 by 5268321
[考研] 材料调剂 +14 一样YWY 2026-04-06 14/700 2026-04-08 23:00 by 猪会飞
[考研] 电子信息346 +4 zuoshaodian 2026-04-08 4/200 2026-04-08 11:54 by zzucheup
[考研] 288环境专硕,求调材料方向 +35 lllllos 2026-04-04 39/1950 2026-04-07 23:24 by 一只好果子?
[考研] 286求调剂 +20 Faune 2026-04-06 20/1000 2026-04-07 11:33 by 诗与自由
[考研] 华南理工0703化学,总分336求调剂 +10 手机用户 2026-04-03 11/550 2026-04-06 22:20 by michael2011
[考研] 求助 +3 卡卡东88 2026-04-06 4/200 2026-04-06 15:28 by going home
[考研] 一志愿C9的化学工程(085602) 340分,感觉校内调剂无望,求调剂 +12 万事宜臻 2026-04-04 12/600 2026-04-06 07:46 by 无际的草原
[考研] 302分 085601求调剂推荐 +11 zyx上岸! 2026-04-05 11/550 2026-04-05 22:13 by dongzh2009
[考研] 08专硕275调剂 +5 AaAa7420 2026-04-05 5/250 2026-04-05 18:01 by jkddd
[考研] 316求调剂 +9 墨辰_Orion926 2026-04-04 9/450 2026-04-04 21:35 by lbsjt
[考研] 材料383求调剂 +5 郭阳阳阳成 2026-04-04 5/250 2026-04-04 19:06 by dongzh2009
信息提示
请填处理意见