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wd519wd706银虫 (小有名气)
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| Transcriptomic and Phenotypic Responses of Listeria monocytogenes Strains Possessing Different Growth Efficiencies under Acidic Conditions。In an experiment delineating aciduric strains, food and clinical Listeria monocytogenes isolates tended to produce the most biomass whereas ovine and avian strains produced comparatively less biomass when exposed to high levels of sodium diacetate (SD) and potassium sorbate. Compared to reference strains that exhibited greater acid sensitivity, representative food isolates with comparatively good growth capacities in the presence of 21 mM SD at pH 5.0 accumulated reduced levels of acetate anion and K+ ion. The aciduric nature of SD-resistant strains was also reflected by comparatively high tolerance to pH 2.4 (HCl) acid challenges, a property boosted by the presence of SD. Exposure to elevated levels of SD (21 mM SD at pH 5.0) was found to have broad effects on gene expression, as differentiated from effects caused by mildly acidic conditions (pH 5.0). SD-resistant strain FW04/0025 was more responsive to elevated SD, increasing the expression of 222 genes (>2-fold change [P < 0.05]), compared to the more sensitive EGD reference strain, which exhibited increases in expression of 112 genes. Key differences between the strains in relation to SD-enhanced transcripts were notably associated with the cell envelope, oxidative stress management, and intermediary metabolism. SD thus appears to differentially influence growth efficiency and survival of strains, under conditions relevant to acidic foods, that could be due to altered cell wall and metabolic phenotypes. |
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sundaes21
木虫 (小有名气)
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发芽土豆(金币+1):Thx for your participation! 2010-07-19 16:45:18
wd519wd706(金币+8, 翻译EPI+1):感谢你 2010-07-19 23:26:21
发芽土豆(金币+1):Thx for your participation! 2010-07-19 16:45:18
wd519wd706(金币+8, 翻译EPI+1):感谢你 2010-07-19 23:26:21
| 转录组学以及表象的单核细胞增多性李氏杆菌株的反应在酸性的条件下具有不同的生长效率。在实验过程中圈定的嗜酸菌株中，当被暴露在高浓度的双乙酸钠和山梨酸钾条件下时，食品和临床中分离的单核细胞增多性李氏杆菌倾向于产生最多的生物质，而ovine菌株和avain菌株则产生较少的生物质。与对照的菌株相比，以上菌株展现出极强的酸性敏感性，其中生长能力相对好的食品的分离株具有代表性，当它在21mMSD，pH=5.0时，醋酸根离子和钾离子含量降低。这种嗜酸的特性同时也可以通过对于pH=2.4的盐酸的高抗拒性表现出来，这种性能正是引入SD所促进的。在较高的SD存在量下（21mM pH=5.0），与温和的酸性条件下比，其对于基因的表达有着广泛的影响。抗SD的菌株 FW04/0025对于较高的SD含量的响应更为强烈，与更为敏感的EGD对照株相比，其增加了222个基因的表达（），而对照株增加了112个基因的表达。普通的转录与SD加强的转录的最重要的不同点与细胞膜，（氧化应激性），中间代谢有着重要联系。因此对于生长效率以及菌株的存活，在酸性食物的条件下，SD呈现出不同的影响特性，这可能是细胞壁以及代谢表型的变化引起的。 |
2楼2010-07-19 14:34:43
zerohead
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3楼2010-07-19 14:37:47
sundaes21
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4楼2010-07-19 14:44:25
