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[求助]
出所有金币,求助翻译一段话
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1、采用酶法水解制备鱼水解物,根据酶解液中游离氨基氮浓度变化进程曲线,比较了Flavourzyme、Alcalase、1398中性蛋白酶这三种酶的水解效果,确定复合风味蛋白酶Flavourzyme为鱼下脚料酶解的工艺用酶。 2、在确定酶解工艺用酶的基础上,应用四因子二次回归正交旋转实验设计,以水解度为指标,深入考察酶用量,pH,温度,时间对水解工艺的影响,并建立了相应的工艺调控数学模型。由模型的方差分析及F检验,确定四因素对水解度影响程度的大小。 3、对模型进一步进行岭脊分析,得出最佳酶水解工艺条件。 4、通过正交实验法,验证了最佳工艺条件下的酶解度,深入考察了酶用量,pH,温度,时间对水解工艺的影响,得出以下结论:水解工艺的最适pH为 7.0,水解适宜温度为54℃,酶用量和时间对水解度的影响呈正相关。 |
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【答案】应助回帖
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sirljz(金币+5):谢谢交流 2010-04-30 22:07
sirljz(金币+5):谢谢交流 2010-04-30 22:07
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1.Prepared by enzymatic hydrolysis of fish hydrolysates, according hydrolyzate concentration of free amino nitrogen in the process of curve,Compared the enzyme hydrolysis effect of Flavourzyme, Alcalase, 1398 the neutral protease,determine flavourzyme Flavourzyme as fish scraps feed enzyme hydrolysis process. 2.Based on the determined enzyme of the enzyme of hydrolysis process,Applicate the four-factor quadratic regression orthogonal rotation experimental design, degree of hydrolysis as an index,In-depth study enzyme dosage, pH, temperature, time of hydrolysis,And creat the corresponding mathematical model of process control,By the model analysis of variance and F test, make sure the impact of four factors on the degree of hydrolysis. 3.the further ridge analysis showed that the optimum conditions for enzymatic hydrolysis. 4.By orthogonal experimental design to verify the optimum conditions of hydrolysis degree,In-depth study of the enzyme dosage, pH, temperature and time on the impact of hydrolysis,Draw the following conclusions: The optimal hydrolysis pH 7.0, optimum temperature for hydrolysis of 54 ℃, enzyme dosage and time on hydrolysis degree of positive correlation. |
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