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Immediately after plants were removed from the spray chamber, four 1-ml droplets of 14C-glyphosate spotting solution were placed on the adaxial surface of the secondright leaflet on the sixth true leaf of each plant during the first run of the experiment, with leaflets on the right and left of the petiole closest to the stem numbered as the first leaflet on the right and the first leaflet on the left, respectively. The 14C-glyphosate spotting solution was prepared by combining 56.3 ml of a 14C-glyphosate solution5 [14C-phosphonomethyl- labelled with 88.8 Bq mmol21 specific activity, . 98% radiochemical purity] with 944 ml of the respective glyphosate spray solution, resulting in an activity of 417 Bq ml21. During the second run of the experiment, the 14C-glyphosate spotting solution was placed on the adaxial surface of the secondright leaflet on the fourth true leaf. Leaves chosen for spotting were located near the top of the plant and would consistently intercept the spray solution. The difference in location of the spotting location between the two runs was due to differences in plant heights (Table 2). Droplets were placed randomly on the leaflet with a digital microsyringe,6 but leaf veins were avoided. |
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- ³æºÅ: 684724
- ×¢²á: 2008-12-30
- ÐÔ±ð: MM
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jean2008(½ð±Ò+20, ·ÒëEPI+1): 2010-04-27 08:18
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