| 查看: 289 | 回复: 3 | |||
| 当前主题已经存档。 | |||
| 【有奖交流】积极回复本帖子，参与交流，就有机会分得作者 小虫之老虫 的 2 个金币 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
[交流]
【求助/交流】求助文献一篇，内附地址链接。
|
|||
|
http://d.wanfangdata.com.cn/NSTLQK_NSTL_QK16431639.aspx Rapid detection and quantification of tyrosine decarboxylase gene (tdc) and its expression in gram-positive bacteria associated with fermented foods using PCR-based methods Journal of Food Protection, 71, 93–101. Torriani, S., Gatto, V., Sembeni, S., Tofalo, R., Suzzi, G., Belletti, N., et al. (2008). |
回复此楼» 猜你喜欢
本科211，报考085601-310分
已经有6人回复
-
RY：中国产出的科学垃圾论文，绝对数量和比例都世界第一
已经有4人回复
-
279学硕食品专业求调剂院校
已经有24人回复
-
求调剂学校
已经有5人回复
-
291 求调剂
已经有34人回复
-
085408光电信息工程专硕355一志愿长春光机所调剂
已经有4人回复
-
材料工程085601，270求调剂
已经有43人回复
-
材料相关专业344求调剂双非工科学校或课题组
已经有19人回复
-
085600材料与化工329分求调剂
已经有21人回复
-
297工科调剂?
已经有8人回复
4楼2010-03-26 10:14:13
chaj
至尊木虫 (著名写手)
生物纳米分析
- 应助: 6 (幼儿园)
- 金币: 12710.3
- 散金: 6
- 红花: 1
- 帖子: 2073
- 在线: 96.3小时
- 虫号: 882802
- 注册: 2009-10-24
- 性别: GG
- 专业: 生化分析及生物传感
★ ★
amisking(金币+2): 2010-03-25 22:04
amisking(金币+2): 2010-03-25 22:04
|
摘要: In this study, PCR-based procedures were developed to detect the occurrence and quantify the expression of the tyrosine decarboxylase gene (tdc) in gram-positive bacteria associated with fermented foods. Consensus primers were used in conventional and reverse transcription PCR to analyze a collection of 87 pure cultures of lactic acid bacteria and staphylococci. All enterococci, Staphylococcus epidermidis, Lactobacillus brevis, Lactobacillus curvatus, and Lactobacillus fermentum strains and 1 of 10 Staphylococcus xylosus strains produced amplification products with the primers DEC5 and DEC3 in accordance with results of the screening plate method and with previously reported result obtained with high-performance liquid chromatography. No amplicons were obtained for tyramine-negative strains, confirming the high specificity of these new primers. A novel quantitative real-time PCR assay was successfully applied to quantify tdc and its transcript in pure cultures and in meat and meat products. This assay allowed estimation of the influence of different variables (pH, temperature, and NaCl concentration) on the tdc expression of the tyraminogenic strain Enterococcus faecalis EF37 after 72 h of growth in M17 medium. Data obtained suggest that stressful conditions could induce greater tyrosine decarboxylase activity. The culture-independent PCR procedures developed here may be used for reliable and fast detection and quantification of bacterial tyraminogenic activity without the limitations of conventional techniques. 文献类型: Proceedings Paper 语言: English KeyWords Plus: LACTIC-ACID BACTERIA; LACTOBACILLUS-BREVIS IOEB-9809; HISTAMINE-PRODUCING BACTERIA; TYRAMINE-PRODUCING BACTERIA; BIOGENIC-AMINE PRODUCTION; ENTEROCOCCUS-FAECALIS; IDENTIFICATION; PURIFICATION; ORGANISMS; HISTIDINE 通讯作者地址: Torriani, S (通讯作者), Dipartimento Sci Technol & Mercoti Vice Vino, Via Pieve 70-37029, Verona, Italy 地址: 1. Univ Verona, Dipartimento Sci & Tecnol, I-37134 Verona, Italy |
2楼2010-03-25 21:53:21
3楼2010-03-25 22:16:45
