| 查看: 359 | 回复: 3 | |||
| 当前主题已经存档。 | |||
| 【有奖交流】积极回复本帖子，参与交流，就有机会分得作者 小虫之老虫 的 2 个金币 | |||
[交流]
【求助/交流】求助文献一篇，内附地址链接。
|
|||
|
http://d.wanfangdata.com.cn/NSTLQK_NSTL_QK16431639.aspx Rapid detection and quantification of tyrosine decarboxylase gene (tdc) and its expression in gram-positive bacteria associated with fermented foods using PCR-based methods Journal of Food Protection, 71, 93–101. Torriani, S., Gatto, V., Sembeni, S., Tofalo, R., Suzzi, G., Belletti, N., et al. (2008). |
回复此楼» 猜你喜欢
关于Filecode分析方法
已经有10人回复
-
重要来源：本周末出结果
已经有9人回复
-
FileCode能看出啥？
已经有24人回复
-
我的国基提前知道中了，可是同事的操作让我实在接受不了，怎么会有这样的人
已经有14人回复
-
结合人工智能，周易传统文化，filecode打分制来了，3分以上希望很大。
已经有4人回复
-
2019年青年基金涵评意见，大家看看几个A，几个B?
已经有11人回复
-
应该是93bebmhtak前后十一个字符比较关键
已经有36人回复
-
不应该看fileCode
已经有6人回复
-
好奇怪的filecode
已经有6人回复
-
filecode
已经有15人回复
chaj
至尊木虫 (著名写手)
生物纳米分析
- 应助: 6 (幼儿园)
- 金币: 12710.3
- 散金: 6
- 红花: 1
- 帖子: 2073
- 在线: 96.3小时
- 虫号: 882802
- 注册: 2009-10-24
- 性别: GG
- 专业: 生化分析及生物传感
★ ★
amisking(金币+2): 2010-03-25 22:04
amisking(金币+2): 2010-03-25 22:04
|
摘要: In this study, PCR-based procedures were developed to detect the occurrence and quantify the expression of the tyrosine decarboxylase gene (tdc) in gram-positive bacteria associated with fermented foods. Consensus primers were used in conventional and reverse transcription PCR to analyze a collection of 87 pure cultures of lactic acid bacteria and staphylococci. All enterococci, Staphylococcus epidermidis, Lactobacillus brevis, Lactobacillus curvatus, and Lactobacillus fermentum strains and 1 of 10 Staphylococcus xylosus strains produced amplification products with the primers DEC5 and DEC3 in accordance with results of the screening plate method and with previously reported result obtained with high-performance liquid chromatography. No amplicons were obtained for tyramine-negative strains, confirming the high specificity of these new primers. A novel quantitative real-time PCR assay was successfully applied to quantify tdc and its transcript in pure cultures and in meat and meat products. This assay allowed estimation of the influence of different variables (pH, temperature, and NaCl concentration) on the tdc expression of the tyraminogenic strain Enterococcus faecalis EF37 after 72 h of growth in M17 medium. Data obtained suggest that stressful conditions could induce greater tyrosine decarboxylase activity. The culture-independent PCR procedures developed here may be used for reliable and fast detection and quantification of bacterial tyraminogenic activity without the limitations of conventional techniques. 文献类型: Proceedings Paper 语言: English KeyWords Plus: LACTIC-ACID BACTERIA; LACTOBACILLUS-BREVIS IOEB-9809; HISTAMINE-PRODUCING BACTERIA; TYRAMINE-PRODUCING BACTERIA; BIOGENIC-AMINE PRODUCTION; ENTEROCOCCUS-FAECALIS; IDENTIFICATION; PURIFICATION; ORGANISMS; HISTIDINE 通讯作者地址: Torriani, S (通讯作者), Dipartimento Sci Technol & Mercoti Vice Vino, Via Pieve 70-37029, Verona, Italy 地址: 1. Univ Verona, Dipartimento Sci & Tecnol, I-37134 Verona, Italy |
2楼2010-03-25 21:53:21
3楼2010-03-25 22:16:45
4楼2010-03-26 10:14:13
