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http://d.wanfangdata.com.cn/NSTLQK_NSTL_QK16431639.aspx Rapid detection and quantification of tyrosine decarboxylase gene (tdc) and its expression in gram-positive bacteria associated with fermented foods using PCR-based methods Journal of Food Protection, 71, 93–101. Torriani, S., Gatto, V., Sembeni, S., Tofalo, R., Suzzi, G., Belletti, N., et al. (2008). |
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chaj
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amisking(金币+2): 2010-03-25 22:04
amisking(金币+2): 2010-03-25 22:04
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摘要: In this study, PCR-based procedures were developed to detect the occurrence and quantify the expression of the tyrosine decarboxylase gene (tdc) in gram-positive bacteria associated with fermented foods. Consensus primers were used in conventional and reverse transcription PCR to analyze a collection of 87 pure cultures of lactic acid bacteria and staphylococci. All enterococci, Staphylococcus epidermidis, Lactobacillus brevis, Lactobacillus curvatus, and Lactobacillus fermentum strains and 1 of 10 Staphylococcus xylosus strains produced amplification products with the primers DEC5 and DEC3 in accordance with results of the screening plate method and with previously reported result obtained with high-performance liquid chromatography. No amplicons were obtained for tyramine-negative strains, confirming the high specificity of these new primers. A novel quantitative real-time PCR assay was successfully applied to quantify tdc and its transcript in pure cultures and in meat and meat products. This assay allowed estimation of the influence of different variables (pH, temperature, and NaCl concentration) on the tdc expression of the tyraminogenic strain Enterococcus faecalis EF37 after 72 h of growth in M17 medium. Data obtained suggest that stressful conditions could induce greater tyrosine decarboxylase activity. The culture-independent PCR procedures developed here may be used for reliable and fast detection and quantification of bacterial tyraminogenic activity without the limitations of conventional techniques. 文献类型: Proceedings Paper 语言: English KeyWords Plus: LACTIC-ACID BACTERIA; LACTOBACILLUS-BREVIS IOEB-9809; HISTAMINE-PRODUCING BACTERIA; TYRAMINE-PRODUCING BACTERIA; BIOGENIC-AMINE PRODUCTION; ENTEROCOCCUS-FAECALIS; IDENTIFICATION; PURIFICATION; ORGANISMS; HISTIDINE 通讯作者地址: Torriani, S (通讯作者), Dipartimento Sci Technol & Mercoti Vice Vino, Via Pieve 70-37029, Verona, Italy 地址: 1. Univ Verona, Dipartimento Sci & Tecnol, I-37134 Verona, Italy |
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