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摘要：为了探讨氟致大鼠肾损伤的机理，让大鼠自由饮用含15mg/L(低氟组)、30mg/L(中氟组)、60mg/L(高氟组)的氟化钠溶液。9个月后处死大鼠，测定其肾脏的氧化水平和核因子κB(NF-κB)的表达量。结果表明,各染氟组大鼠肾脏出现明显的组织病理学形态改变，高氟组体重显著低于对照组。与对照组相比，低氟、高氟组MDA含量显著提高；低氟组谷胱甘肽过氧化物酶活(GSH-Px)极显著上升(P<0.01)，高氟组显著上升(P<0.05)；各染氟组超氧化物歧化酶(SOD)活性虽有下降趋势，但未见显著差异。随着染毒剂量的加大，NF-κB的表达量呈上升趋势，高氟组显著高于对照组(P<0.05)。结果提示，慢性氟中毒可引起大鼠肾脏抗氧化酶活性降低，使脂质过氧化物增多，NF-κB表达水平上升，引起肾脏组织损伤。肾脏组织内NF-κB对氟中毒较敏感，可以作为诊断氟致肾脏损伤的分子指标之一。 Abstract: In order to study the fluoride on the rat kidney tissue toxicity, let the rats to drink freely the water containing 15, 30, and 60mg /L of sodium fluoride. These rats were put to death after 9 months, the oxidation level and nuclear factor κB (NF-κB) expression level in kidney were determined. The results showed that fluoride poisoned rat kidney in each group showed a visible morphological morphological change, high dose group body weight was lower obviously than the control group, the MDA contents of low dose, high dose groups obviously higher than the control group. The enzyme of GSH-Px activity of low dose group obviously higher than the control group, high dose group increased significantly. As to SOD activity, there were trends of increase but no obviously difference. As the exposure dose increased, NF-κB expression level was also on the rise, high dose group obviously higher than the control group. The study indicated that Fluoride can decrease the activity of kidney antioxidant enzymes in rat, resulting in an increase in lipid peroxidation, NF-κB expression level increased, causing tissue damage. NF-κB in kidney is sensitive to fluorosis, which can be one of molecular diagnostic makers for kidney damage by fluoride. |
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sirljz(金币+2):谢谢交流 2010-03-25 19:52
112976553(金币+10):不错哈 2010-03-26 10:09
sirljz(金币+2):谢谢交流 2010-03-25 19:52
112976553(金币+10):不错哈 2010-03-26 10:09
| Abstract: In order to study the toxicity of fluoride on rat’s kidney tissue, the polluted water containing 15, 30, and 60mg /L of sodium fluoride was fed to rats in a natural way. The rats were put to death after 9 months. The oxidation level and nuclear factor κB (NF-κB) expression level in rat’s kidney were determined. The results showed a visible morphological change among each group of fluoride poisoned rat’s kidneys and the body weights of high dosed group were lower greatly than the controlled group. Both MDA contents of low dosed and high dosed groups were higher than the controlled group. The enzyme activities of GSH-Px of low dosed group and the high dosed group were obviously higher than the controlled group. Although there were increasing trends of SOD activity, the difference is not considerable. NF-κB expression level was rising as the exposure dose increased and that of high dosed group was dramatically higher than the controlled group. The study indicated that Fluoride could decrease the activity of antioxidant enzymes in rat’s kidney and increase lipid peroxidation and NF-κB expression level, which caused the damage of kidney tissue. NF-κB in kidney is sensitive to fluorosis, which can be taken as one of molecular diagnostic markers for fluoride damaged kidneys. |
2楼2010-03-25 18:29:15
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all rats exposed to fluoride showed visible histopathological renal morphological changes, high-dose group body weight was lower obviously(P<0.05), the MDA contents of low-dose, high-dose groups were obviously increased(P<0.05). The enzyme of GSH-Px activity of low-dose group obviously higher than the control group (P<0.05), high dose group increased significantly (P<0.01). As to SOD activity, although a downward trend, but no obviously difference. As the exposure dose increased, NF-κB expression level was also on the rise, high-dose group obviously higher than the control group (P<0.05). The results indicated that chronic fluorosis can reduce the activity of kidney antioxidant enzymes in rat, resulting in an increase in lipid peroxidation, NF-κB expression level increased, caused kidney tissue damage. NF-κB in kidney tissue is sensitive to fluorosis, which can be one of molecular diagnostic makers for kidney damage by fluoride. 这一段看大虾们还能不能再改改，前面定下来不动了。 |
3楼2010-03-26 10:42:09
pharmjackie
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112976553(金币+10): 2010-03-26 18:19
| …… body weight [was lower］(lowered，decreased) obviously(P<0.05), the MDA contents of low-dose, high-dose groups [were obviously](建议删除) increased (significantly) (P<0.05). The enzyme of GSH-Px activity of low-dose group [](应添加was)obviously higher than the control group (P<0.05), (with)high dose group increased significantly (P<0.01). As to SOD activity, [although a downward trend, but no obviously difference] (a downward but not statistically different trend was observed). [As the exposure dose increased, NF-κB expression level was also on the rise](NF-κB expression level increased with increasing exposure dose), with high-dose group obviously higher than the control group (P<0.05). The results indicated that chronic fluorosis can reduce the activity of kidney antioxidant enzymes in rat, resulting in an increase in lipid peroxidation, [NF-κB expression level increased, caused kidney tissue damage. ]没有看懂这一句。［NF-κB in kidney tissue is sensitive to fluorosis, which can be one of molecular diagnostic makers for kidney damage by fluoride.］没有看懂 |
4楼2010-03-26 13:01:52