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Ibuprofen Inhibits Skeletal Muscle Hypertrophy in Rats Abstract Purpose: We sought to determine whether cyclooxygenase (COX) activity is necessary for overload-induced growth of adult rat skeletal muscle, and whether nitric oxide synthase (NOS) activity is involved in upregulation of COX messenger RNA (mRNA) expression in skeletal muscle. Methods: Unilateral surgical removal of the gastrocnemius and soleus was performed on the right hindlimb of 16 female Sprague-Dawley rats (~230 g) to induce chronic overload (OL) of the plantaris for 14 d, with sham surgeries performed on the contralateral leg as a normally loaded (NL) control. Half of the rats were treated with the nonspecific COX inhibitor, ibuprofen (0.2 mg•mL-1 in drinking water; ~20 mg•kg-1•d-1). In a second experiment, the plantaris was unilaterally overloaded for 5 or 14 d in male rats (~350 g; N = 16 rats per time point) and half of the animals were treated with the NOS inhibitor, L-NAME (0.75 mg•mL-1 in drinking water; ~90 mg•kg-1•d-1). Results: Ibuprofen treatment inhibited plantaris hypertrophy by approximately 50% (P < 0.05) following 14 d of OL, as did L-NAME treatment (P < 0.05). COX-1 and COX-2 mRNA did not differ between any groups at 5 d. At 14 d, however, L-NAME caused a 30-fold increase in plantaris COX-1 mRNA expression independent of loading condition. Additionally, OL induced a 20-fold increase in COX-2 mRNA expression compared with NL (P < 0.05) at 14 d, without affecting COX-1 mRNA level. L-NAME treatment significantly inhibited OL-induced expression of COX-2 mRNA. Conclusion: COX activity is important for in vivo muscle hypertrophy, and plantaris overload is associated with NOS activity-dependent COX-2 expression. |
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yyat(金币+10):谢谢 2010-03-30 08:29
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试译如下：不甚严谨，不甚正确，请专业人士指正： 布洛芬在鼠中抑制骨骼肌肥大作用研究 摘要：我们研究了环氧合酶（COX）的活性对于成年鼠骨骼肌超负荷诱导的的生长是否必须，一氧化氮合成酶(NOS)是否在COX的mRNA在骨骼肌表达的上调中发挥作用。 方法：对16只雌性Sprague-Dawley鼠（约230克）的右后肢进行单侧手术移除腓肠肌和比目鱼肌，以使内诱导跖肌慢性超负荷（OL）14天，对其对侧肢体作假手术以作为正常负荷的对照（NL）。半数鼠使用非特异性COX抑制剂，布洛芬（于其饮水中加入0.2mg/ml，约20mg/kg.天）。另一项实验中，使雄鼠跖肌单侧超负荷5或14天（约350克，每时间点样本数16只），其中半数动物使用NOS,L-NAME(饮水中加入0.75mg/ml，约90mg/Kg.天。 结果：14天OL后使用布洛芬治疗跖肥抑制率大约50%（P<0.05)，与L-NAME相似（P<0.05).两组在第5天时的COX-1和COX-2 mRNA并没有显著差异。然而在第14天时，L-NAME引起跖肌COX-1 mRNA表达增长了30倍，且与负荷无关。此外，与NL相比，在第14天时OL引起COX-2 mRNA表达增长20倍（P<0.05)，且没有影响COX-1 mRNA水平。使用L-NAME治疗显著抑制了OL-诱导的COX-2 mRNA表达。 结论： COX活性在体内骨骼肌肥大中起着重要作用。跖肌超负荷是与NOS活性依赖的COX-2表达关系密切。 |
2楼2010-03-22 19:37:44
