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wittyboy

至尊木虫 (著名写手)

[求助] 帮忙看一段审稿人的意见--谢谢

The use of dry weight measurements for quantification of the stock solutions is a crude and in-accurate means of protein quantification. Often times in commercially purchased peptides greater than 10% of the dry weight is constituted from salts and residual water (the % purity values indicated by the manufacturers relate to what % of all peptide material in the vial is the target peptide - it does not indicate what percent of all dry weight is the target peptide).

我是用标准物质配置标准溶液建立标准曲线
然后对粗蛋白质样品中的这个标准物质进行定量

1.审稿人为什么说我是标准溶液的定量啊?
2.无论样品组成如何,只要是我建立的串联质谱方法合适,就可以进定量啊
和杂质无关啊
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bear2007

铁杆木虫 (著名写手)

wittyboy(金币+2):谢谢 2010-03-31 22:13
The use of dry weight measurements for quantification of the stock solutions is a crude and in-accurate means of protein quantification.

称干物质配置标准比较粗略,在蛋白质定量中不精确
Often times in commercially purchased peptides greater than 10% of the dry weight is constituted from salts and residual water
通常购买的PEp中超过10%的是盐和水分
(the % purity values indicated by the manufacturers relate to what % of all peptide material in the vial is the target peptide - it does not indicate what percent of all dry weight is the target peptide).

商品的纯度指的是所有peptide中含所含有目的pep的百分比(target peptide ),而不是净重中含有目的pep的百分比。

净重*  X% 要大于目的pep含量。因为peptide种类也较多吧,呵呵
就像 氯化钠氯化钾冰棍(氯化钠纯度98%,应该是指占总盐的比例),你要计算食盐的含量,你要除去水,还要除去氯化钾

[ Last edited by bear2007 on 2010-3-24 at 14:13 ]
腾冲天好水好玉更好
5楼2010-03-24 14:09:51
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l348704462

金虫 (小有名气)

要好好复习英语
2楼2010-03-24 09:48:56
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liuheguang

新虫 (小有名气)

wittyboy(金币+1):谢谢 2010-03-31 22:13
他说用干重法定量标准液(原液)的方法是不精确的,你说你是标准物质配置标准的溶液哈,这点应该没说错吧。后面他说买的缩氨酸里面超过的百分之十的干重是来自盐和残留的水分,他的意思应该是要考虑杂质吧,至于你说的串联质谱我就不懂了,你可以再仔细看看
思想上大手大脚,生活上适可而止
3楼2010-03-24 13:15:02
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wittyboy

至尊木虫 (著名写手)

我觉得应该是说我没有采用基质校正来做标准溶液吧
4楼2010-03-24 13:30:28
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