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yzdjyj铁杆木虫 (正式写手)
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求助翻译一段农业方面的中译英，悬赏100金币！关于棉花转基因方面的！
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2.1 棉花蔗糖合酶新基因GhSUSA1的克隆鉴定和序列分析 根据TAIL技术，经过三次5’扩增和两次3’扩增后经测序获得5582bp基因组片段，并且经过RT-PCR扩增测序得到了2660bp的cDNA片段，包含了完整ORF、5’-UTR和3’-UTR。 以此基因ORF的推断氨基酸序列经BLAST(http://www.ncbi.nlm.nih.gov/blast)比对，发现其与柑橘蔗糖合酶的氨基酸序列具有最高同源性（87%），故将其命名为GhSUSA1。GhSUSA1 ORF全长2430bp，共编码809个氨基酸，由于基因组序列的获得，基因结构分析（http://www.dnastar.com）该基因由15个外显子和14个内含子组成，从内含子大小来看更类似于单子叶植物的蔗糖合酶基因结构，推测其内含子对其功能有一定作用。 该基因由蔗糖合成结构域和糖基转移结构域组成，用Expasy pI/Mw程序（http://us.expasy.org/）对GhSUSA1的氨基酸序列进行了一级结构的预测，其理论上的等电点为5.97，分子量为92117.31Da。 通过半定量RT-PCR分析，GhSUSA1基因在陆地棉不同组织器官中组成性表达，其中叶片和萼片中表达量最低，在15d纤维中表达量没有0d胚珠中高。定量RT-PCR分析开花后不同时期胚珠及纤维中GhSUSA1表达量，发现纤维发育不同时期表达量有差异，起始期表达量高于生长期，开花后25d表达量高于其他时期，这正是次生壁发育时期。Southern blot技术分析该基因在陆地棉中存在2个拷贝，由于陆地棉是异源四倍体，所以推测A、D亚组各存在一个拷贝。 |
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yzdjyj
铁杆木虫 (正式写手)
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2楼2010-03-02 12:38:48
clearsky7228
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yzdjyj(金币+50):谢谢，写的还可以！ 2010-03-03 15:18
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2.1 棉花蔗糖合酶新基因GhSUSA1的克隆鉴定和序列分析 根据TAIL技术，经过三次5’扩增和两次3’扩增后经测序获得5582bp基因组片段，并且经过RT-PCR扩增测序得到了2660bp的cDNA片段，包含了完整ORF、5’-UTR和3’-UTR。 2.1 The cloning identification and sequence analysis of cotton sucrose synthase new genes GhSUSA1 According to TAIL technology, after three 5 'amplification and two 3' amplified one 5582bp genomic fragment was gained by sequencing , and 2660bp cDNA fragment was gained by RT-PCR amplification sequencing , which was containing complete ORF, 5'-UTR and the 3'-UTR. 以此基因ORF的推断氨基酸序列经BLAST(http://www.ncbi.nlm.nih.gov/blast)比对，发现其与柑橘蔗糖合酶的氨基酸序列具有最高同源性（87%），故将其命名为GhSUSA1。 Through BLAST (http://www.ncbi.nlm.nih.gov/blast)comparison,it is found that the inferred amino acid sequences from this gene ORF had the highest homology (87%) with citrus sucrose synthase amino acid sequence .Therefore, it was named as GhSUSA1. GhSUSA1 ORF全长2430bp，共编码809个氨基酸，由于基因组序列的获得，基因结构分析（http://www.dnastar.com）该基因由15个外显子和14个内含子组成，从内含子大小来看更类似于单子叶植物的蔗糖合酶基因结构，推测其内含子对其功能有一定作用。 The length of GhSUSA1 ORF is 2430bp, encoding 809 amino acids.Due to the acquisition of genome sequence, gene structure analysis showed(http://www.dnastar.com) the gene consisting a composition of 15 exons and 14 introns . From the view of intron size ,it is more similar to the sucrose synthase gene structure of monocotyledons, and speculated its introns have a certain role in its function. |
3楼2010-03-03 14:32:39
clearsky7228
金虫 (正式写手)
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yzdjyj(金币+100):谢谢你！就你一个人回复你，把金币全给你了！ 2010-03-03 15:24
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The gene is composed by sucrose synthesis structure domain and glycosyl transferase structure domain .The primary structure predicted of amino acid sequences of GhSUSA1 was taken with the Expasy pI / Mw program (http://us.expasy.org/) ,which indicated its theoretical isoelectric point was 5.97, a molecular weight was 92117.31Da. By semi-quantitative RT-PCR analysis, GhSUSA1 genes were constitutive expressed in different tissues of upland cotton , in which expression number in leaves and sepals was the lowest while expression number in the 15d fibers was less than that of in 0d ovules. Quantitative RT-PCR analysis to GhSUSA1 expression number in different times ovule and fiber after flowering found that the expression number of fiber development in different periods were different, that is the expression in starting period was above that in the growing period,expression number in 25d after flowering higher than that in other periods, which was the time for Health-wall development stages. The gene contains two copies in upland cotton by Southern blot technology analysis. Because upland cotton is allotetraploid, it is speculated that there is a copy existing in A, D sub-group,respactively. |
4楼2010-03-03 15:02:06
