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lkd

铜虫 (小有名气)

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在线: 36.5小时
虫号: 770378
注册: 2009-05-14
性别: GG
专业: 色谱分析

[交流] 一段实验方法的翻译！

2.2. Preparation of dispersions
b-lg-Enriched powder was dispersed (6%, w/w) in
distilled/deionised water or in a 100mM NaCl solution
with stirring at room temperature. b-lg-Stabilised emulsions
were prepared for zeta potential determination. A
pre-emulsion of 1% b-lg and 20% rapeseed oil (Spar Ltd.,
Dublin 12, Ireland) was prepared at pH values of 7.0 and
3.0 using a using a Silverson L4RT mixer (Silverson
Machines Ltd., Waterside, Chesham, Bucks, England) for
10 min at 5000 rpm. The mixture was homogenised using a
Microfluidizers M-110 EH Processor (Microfluidiser
Corporation, 68623 Lampertheim, Germany) at 22 1C
and 1500 bar. The emulsions formed at pH 7.0 and 3.0
had particle size values (D4,3 values) of 282724 and
9737106 nm, respectively. Emulsions with intermediate
pH values were prepared by combining the emulsions
prepared at pH 7.0 and 3.0 in various ratios. Chitosan (1%,
w/w) was dissolved by dispersing in water at 22 1C by
adjusting the pH to 3.0 using 1M HCl. Subsequent pH
adjustment was performed with 1M NaOH. Mixtures of
1% (w/w) b-lg in water or 100mM NaCl, or 1% (w/w) b-lg
and 0.1% (w/w) chitosan in water or 100mM NaCl were
prepared by combining stock solutions (6%, w/w, b-lg;
1%, w/w, chitosan; 500mM NaCl; water) at pH values
ranging from 3.0 to 8.0.

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