| 查看: 243 | 回复: 2 | |||
| 当前主题已经存档。 | |||
[交流]
一段实验方法的翻译！
|
|||
|
2.2. Preparation of dispersions b-lg-Enriched powder was dispersed (6%, w/w) in distilled/deionised water or in a 100mM NaCl solution with stirring at room temperature. b-lg-Stabilised emulsions were prepared for zeta potential determination. A pre-emulsion of 1% b-lg and 20% rapeseed oil (Spar Ltd., Dublin 12, Ireland) was prepared at pH values of 7.0 and 3.0 using a using a Silverson L4RT mixer (Silverson Machines Ltd., Waterside, Chesham, Bucks, England) for 10 min at 5000 rpm. The mixture was homogenised using a Microfluidizers M-110 EH Processor (Microfluidiser Corporation, 68623 Lampertheim, Germany) at 22 1C and 1500 bar. The emulsions formed at pH 7.0 and 3.0 had particle size values (D4,3 values) of 282724 and 9737106 nm, respectively. Emulsions with intermediate pH values were prepared by combining the emulsions prepared at pH 7.0 and 3.0 in various ratios. Chitosan (1%, w/w) was dissolved by dispersing in water at 22 1C by adjusting the pH to 3.0 using 1M HCl. Subsequent pH adjustment was performed with 1M NaOH. Mixtures of 1% (w/w) b-lg in water or 100mM NaCl, or 1% (w/w) b-lg and 0.1% (w/w) chitosan in water or 100mM NaCl were prepared by combining stock solutions (6%, w/w, b-lg; 1%, w/w, chitosan; 500mM NaCl; water) at pH values ranging from 3.0 to 8.0. |
回复此楼» 猜你喜欢
求环氧树脂研发1名
已经有10人回复
-
280求调剂
已经有5人回复
-
什么是人一生最重要的？
已经有10人回复
-
面上可以超过30页吧？
已经有13人回复
-
网上报道青年教师午睡中猝死、熬夜猝死的越来越多，主要哪些原因引起的？
已经有10人回复
-
为什么中国大学工科教授们水了那么多所谓的顶会顶刊，但还是做不出宇树机器人？
已经有13人回复
-
版面费该交吗
已经有17人回复
-
【博士招生】太原理工大学2026化工博士
已经有8人回复
2楼2009-12-29 12:15:51
3楼2009-12-29 12:32:09