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Reductive amination method usually requires a certain reacting tempreture to be carried out. Therefore, three reaction groups were set up here to explore a better temperature condition, the temperature is 50 ¡æ, 60 ¡æ and 70 ¡æ, respectively. Other reaction conditions remain unchanged. The results were showed in Fig. 3. 3A and 3B showed that at 50 ¡æ and 60 ¡æ, the lactose ion peak 365[M+Na]+ and non-reductive amination product ion peak 440[M+Na]+ coexistence; And at 70 ¡æ, Fig. 3C, lactose ion peak 365[M+Na]+ and non-reducive amination product ion peak 440[M+Na]+ have disappeared. Experiments showed that 70 ¡æ is the best temperature for glycans being labeled with aniline.
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yjhorn

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twtilu(½ð±Ò+6,VIP+0): 12-13 20:44
¼òµ¥ÐÞ¸ÄÁËһϣ¬Reductive amination method usually requires a certain reacting£¨Ó¦Îªreaction£© tempreture to be carried out. Therefore, three reaction groups were set up£¨Í¨³£ÓÃdivided£© here to explore£¨figure out ½ÏºÃ£© a better£¨»»³Éan optimal£© temperature condition(¿ÉÒÔÈ¥µô), the temperature is 50 ¡æ, 60 ¡æ and 70 ¡æ, respectively. Other reaction conditions remain unchanged. The results were showed(shown) in Fig. 3. 3A and 3B showed that at 50 ¡æ and 60 ¡æ, the lactose ion peak 365[M+Na]+ and non-reductive amination product ion peak 440[M+Na]+ coexistence(Ó¦¸ÃÓö¯´Êcoexisted); And at 70 ¡æ, Fig. 3C, lactose ion peak 365[M+Na]+ and non-reducive amination product ion peak 440[M+Na]+ have disappeared£¨Ó¦¸ÃÈ¥µôHave£©. Experiments showed that 70 ¡æ is the best£¨»»³Éoptimal£© temperature for glycans being£¨È¥µô£©labeled with aniline.
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