| 查看: 242 | 回复: 3 | |||
| 当前主题已经存档。 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
snoopyzxx木虫 (著名写手)
|
[交流]
【求助/交流】求助：wiley文献一篇
|
||
|
题目：Mechanism studies of coomassie blue and silver staining of proteins 全文地址：http://www3.interscience.wiley.com/journal/113340072/abstract 作者：Miriam R. de Moreno, Jean F. Smith, Robert V. Smith * 谢谢。 |
回复此楼» 猜你喜欢
湖北师范大学招调剂啦
已经有9人回复
-
邵阳学院食品与化学工程学院硕士调剂
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有119人回复
-
天津商业大学 生物与医药课题组招生
已经有0人回复
-
生物化工学硕招收调剂
已经有0人回复
-
广东石油化工学院2026年硕士研究生需要多名生物，制药工程，食品专业的调剂生
已经有0人回复
-
化工申博
已经有3人回复
-
申博自荐
已经有2人回复
-
广西民族大学化学化工学院化学工程与技术学硕，材料化工专硕调剂名额充足！
已经有0人回复
snoopyzxx
木虫 (著名写手)
- MolEPI: 4
- 应助: 49 (小学生)
- 金币: 2994.6
- 散金: 1276
- 红花: 43
- 帖子: 2258
- 在线: 277.2小时
- 虫号: 548497
- 注册: 2008-04-19
- 性别: MM
- 专业: 全球变化生态学
4楼2009-12-12 16:27:44
★ ★
snoopyzxx(金币+2,VIP+0):谢谢支持。 12-12 16:28
snoopyzxx(金币+2,VIP+0):谢谢支持。 12-12 16:28
|
Article Mechanism studies of coomassie blue and silver staining of proteins Miriam R. de Moreno, Jean F. Smith, Robert V. Smith * Drug Dynamics Institute, College of Pharmacy, University of Texas at Austin, Austin, TX 78712-1074 *Correspondence to Robert V. Smith, Drug Dynamics Institute, College of Pharmacy, University of Texas at Austin, Austin, TX 78712-1074 Funded by: Hoffmann-LaRoche Inc., Nutley, NJ Universidad Central de Venezuela Abstract A relatively high complexation affinity has been found for coomassie blue G-250 and the following amino acids: arginine; tyrosine; lysine; and histidine. A linear relationship was observed between log molar absorptivity and log molecular weight of 52 of 69 proteins, polypeptides, and di- and tripeptides that were allowed to react with coomassie blue G-250 in solution. The solution complexation results were used in a study of the detection of the following model proteins: bovine serum albumin, lysozyme, recombinant DNA derived human insulin, and calmodulin. Interactions between coomassie blue stained gels and silver detection reagents were determined and used as the basis for studies of enhanced sensitivity of detection of electrophoretically developed proteins. Sensitivity enhancements of up to eight-fold were observed when various sulfonic acid dye complexed proteins were detected with silver reagents versus the use of silver reagents alone. A site-directed nucleation of silver caused by the protein complexed sulfonic acid dyes is proposed as a mechanism for the observed enhancements. |
2楼2009-12-12 13:09:43
qiang84zhang
银虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 444.3
- 帖子: 475
- 在线: 2.3小时
- 虫号: 895117
- 注册: 2009-11-06
- 性别: GG
- 专业: 生物化学与分子生物学
3楼2009-12-12 14:46:31
