| 查看: 214 | 回复: 3 | |||
| 当前主题已经存档。 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
snoopyzxx木虫 (著名写手)
|
[交流]
【求助/交流】求助：wiley文献一篇
|
||
|
题目：Mechanism studies of coomassie blue and silver staining of proteins 全文地址：http://www3.interscience.wiley.com/journal/113340072/abstract 作者：Miriam R. de Moreno, Jean F. Smith, Robert V. Smith * 谢谢。 |
回复此楼» 猜你喜欢
筑牢营养安全线：以精准检测，护健康基石
已经有0人回复
-
推荐一些20种氨基酸检测的实际应用案例
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有147人回复
-
不合理蛙科研实验中的趣事：实验器材的 “乌龙”
已经有0人回复
-
不合理蛙科研实验中的趣事：和实验材料的 “斗智斗勇”
已经有0人回复
-
蛋白质检测：精准分析，解锁生物分子的密码
已经有0人回复
-
不合理蛙科研实验之小鼠实验：严谨设计，解析生命机制的重要载体
已经有0人回复
-
不合理蛙科研实验之重金属检测：精准筛查，守护健康与环境的防线
已经有0人回复
-
不合理蛙科研实验之“蛙测重金属我背锅三千”
已经有0人回复
-
不合理蛙科研实验之“鼠逃三次我发三篇SCI”
已经有0人回复
qiang84zhang
银虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 444.3
- 帖子: 475
- 在线: 2.3小时
- 虫号: 895117
- 注册: 2009-11-06
- 性别: GG
- 专业: 生物化学与分子生物学
3楼2009-12-12 14:46:31
★ ★
snoopyzxx(金币+2,VIP+0):谢谢支持。 12-12 16:28
snoopyzxx(金币+2,VIP+0):谢谢支持。 12-12 16:28
|
Article Mechanism studies of coomassie blue and silver staining of proteins Miriam R. de Moreno, Jean F. Smith, Robert V. Smith * Drug Dynamics Institute, College of Pharmacy, University of Texas at Austin, Austin, TX 78712-1074 *Correspondence to Robert V. Smith, Drug Dynamics Institute, College of Pharmacy, University of Texas at Austin, Austin, TX 78712-1074 Funded by: Hoffmann-LaRoche Inc., Nutley, NJ Universidad Central de Venezuela Abstract A relatively high complexation affinity has been found for coomassie blue G-250 and the following amino acids: arginine; tyrosine; lysine; and histidine. A linear relationship was observed between log molar absorptivity and log molecular weight of 52 of 69 proteins, polypeptides, and di- and tripeptides that were allowed to react with coomassie blue G-250 in solution. The solution complexation results were used in a study of the detection of the following model proteins: bovine serum albumin, lysozyme, recombinant DNA derived human insulin, and calmodulin. Interactions between coomassie blue stained gels and silver detection reagents were determined and used as the basis for studies of enhanced sensitivity of detection of electrophoretically developed proteins. Sensitivity enhancements of up to eight-fold were observed when various sulfonic acid dye complexed proteins were detected with silver reagents versus the use of silver reagents alone. A site-directed nucleation of silver caused by the protein complexed sulfonic acid dyes is proposed as a mechanism for the observed enhancements. |
2楼2009-12-12 13:09:43
snoopyzxx
木虫 (著名写手)
- MolEPI: 4
- 应助: 49 (小学生)
- 金币: 3232.6
- 散金: 1266
- 红花: 43
- 帖子: 2256
- 在线: 276.6小时
- 虫号: 548497
- 注册: 2008-04-19
- 性别: MM
- 专业: 全球变化生态学
4楼2009-12-12 16:27:44