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【求助/交流】我要IC50值，怎么设计实验？
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我做毒性检测实验，需要得到IC50值，一直迷迷糊糊的，别人告诉我的公式，我觉得有问题，大家觉得该怎么设计实验？怎么计算IC50值的？ 下面这样设计对吗?看不懂 The limiting current of UMEA showed stable and amplified sig- nal for less than 10 s. The concentrations of ferrocyanide that was produced by the reduction of ferricyanide were directly estimated by chronoamperograms. Furthermore, the limiting current can be converted to equivalent inhibitory percentage values following Eq. (1): %inhibition =[1 −(ilim(toxicant) − ilim(t−control))/(ilim(p−control) − ilim(n−control))]× 100 (1) where: ilim (toxicant): output of limiting current at a appropriate con- centration of toxicant and standard GGA198 solution; ilim (t-control): output of limiting current at a appropriate concentration of toxi- cant andnoGGA198 solution;[quote][quote] ilim (p-control): output ofpositive control current; ilim (n-control): output of negative control current. |
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2楼2009-12-11 10:35:01