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根据我们的构想，首先用A作为模板合成分子印迹聚合物来识别B标记的A，发现其识别能力很强，但对各种物质的识别选择性较差。有趣的是，用C作为模板合成的MIP对B标记的A的识别选择性不但大于其它氨基酸的识别能力，而且还远大于B标记的D的识别能力。 我们还试验用B标记的A作为模板合成MIP，用于识别B标记的A，其识别选择性更差，这是由于以A为模板分子合成出来的MIP空腔比较小, 避免了非特异性吸附, 使它在识别B标记的A时特异性有所增强, 又由于识别后发荧光的B部分被置于MIP空腔以外, 更容易与TCPO-H2O2-咪唑产生化学发光, 因而响应速度和灵敏度也有所增强. 这一新方法设计扩大了分析应用范围, 降低了分析成本. |
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cpp19840911(金币+6,VIP+0): 12-9 23:25
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| According to ours conception, first uses A to distinguish B mark as the template synthesis member signature polymer A, discovered that its recognition capability is very strong, but is bad to each material's recognition selectivity. What is more, not only MIP which synthesizes as the template is bigger than other amino acids with C to the B mark A recognition selectivity recognition capability, moreover also far is bigger than B mark D the recognition capability. We also experiment A which marks with B as the template to synthesize MIP, uses in distinguishing B mark A, its recognition selectivity is worse, this is because as the MIP cavity which the template member synthesizes is quite small take A, has avoided the non-specificity adsorption, enables it when distinguishes B mark The specificity to have the enhancement, also, because after distinguishing, the luminescence B part is put in outside the MIP cavity, is easier and the TCPO-H2O2- imidazoleHas the chemiluminescence, thus the speed of response and the sensitivity also have the enhancement. This new methods design expanded the analysis application scope, reduced the analysis cost |
2楼2009-12-07 20:06:33