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fjtcm8772

金虫 (小有名气)

[交流] 【求助】用还原能力评价抗氧化活性问题

还原能力过程如下:
Various concentrations of chestnut extracts (2.5 ml)
were mixed with 2.5 ml of 200 mM sodium phosphate buf-
fer (pH 6.6) and 2.5 ml of 1% potassium ferricyanide. The
mixture was incubated at 50 C for 20 min. After 2.5 ml of
10% trichloroacetic acid (w/v) was added, the mixture was
centrifuged at 1000 rpm for 8 min (Centorion K24OR-2003
refrigerated centrifuge). The upper layer (5 ml) was mixed
with 5 ml of deionised water and 1 ml of 0.1% of ferric
chloride, and the absorbance was measured spectrophoto-
metrically at 700 nm (Barros et al., 2007). The extract con-
centration providing 0.5 of absorbance (EC50) was
calculated from the graph of absorbance at 700 nm against
extract concentration. BHA and a-tocopherol were used as
standards.
请问你们离心后会分层吗?我做的不会分层,还是本来就不会分层,但是文献上又说是上清液?
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shiweidong7727

铜虫 (小有名气)

★ ★
小木虫(金币+0.5):给个红包,谢谢回帖交流
hawkyin(金币+1,VIP+0):谢谢回帖 11-30 22:01
你离心一下,如果没有浑浊的就直接用
5楼2009-11-26 12:53:27
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siwei2020

金虫 (小有名气)

★ ★ ★
小木虫(金币+0.5):给个红包,谢谢回帖交流
hawkyin(金币+1,VIP+0):谢谢回帖交流,常来食品版!~ 11-22 23:05
axi8383(金币+1,VIP+0):谢谢回帖交流, 11-23 05:44
我做还原力测定的时候也没有出现分层现象
2楼2009-11-22 17:26:31
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guodong1997

银虫 (小有名气)

★ ★ ★
小木虫(金币+0.5):给个红包,谢谢回帖交流
hawkyin(金币+1,VIP+0):谢谢回帖交流,常来食品版!~ 11-22 23:05
axi8383(金币+1,VIP+0):谢谢回帖交流, 11-23 05:44
离心不会分层,但有时候会有混浊出现,所以需要离心取上清
3楼2009-11-22 19:07:28
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tracy-1986

新虫 (初入文坛)


小木虫(金币+0.5):给个红包,谢谢回帖交流
我做的也是有些浑浊,可是离心了之后也没有分开啊,还是有些浑浊的,请问是怎么回事,影响结果吗
4楼2009-11-26 10:26:59
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