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biogefart

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[交流] 谁能帮我解释一下?

谁能帮我解释一下?
I presume 40 micrograms of protein, no determination of enzyme concentration is given, just total protein content which is roughly the PM of the obtained recombinant proteins? Are they oligomeric proteins,at least a Western blotting would have been useful.
In addition, one unit of activity is one unit by definition, and the measured unitS are proportional then proportional to the enzime content of the sample.

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yzhang1986

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zap65535(金币+6,VIP+0):补发 12-22 01:12
假定有40mg粗提的复合蛋白,酶浓度没有给出。它们是寡聚蛋白,至少western杂交是有用的。
另外,一单位活力定义为一个单位,测定的酶活力与样本中酶含量成比例
2楼2009-11-13 12:08:34
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yjhorn

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zap65535(金币+6,VIP+0):补发 12-22 01:12
这样会好理解一点:
I presume 40 micrograms of protein, no determination of enzyme concentration is given,just total protein content which is roughly the PM of the obtained recombinant proteins?
可以换成if no determination of enzyme concentration is given,
I presume 40 micrograms of protein,or just total protein content which is roughly the PM of the obtained recombinant proteins?

Are they oligomeric proteins,at least a Western blotting would have been useful.
换成 if they are oligomeric proteins,at least a Western blotting would have been useful.


In addition, one unit of activity is one unit by definition, and the measured unitS are proportional then proportional to the enzime content of the sample.
认识大家是缘分啊!
3楼2009-11-13 12:08:53
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biogefart

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这样的人怎么会做审稿人?前言不搭后语,难道是火星人?
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4楼2009-11-13 12:45:15
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