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yunfeitian3525木虫 (正式写手)
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钱永健发的一篇引用次数超过13000的文章，大家参考一下
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A New Generation of Ca2+ Indicatorsw ith Greatly Improved Fluorescence Properties* Grzegorz Grynkiewicz$, Martin Poenie, and Roger Y. TsienB From the Department of Physiology-Anatomy, Uniuersitoyf California, Berkeley, California9 4720 A new family of highly fluorescent indicators has been synthesized forbiochemical studies of the physiological role of cytosolic free Ca2+.The compounds combine an 8-coordinate tetracarboxylate chelating sitewith stilbene chromophores. Incorporation of the ethylenic linkage ofthe stilbene into a heterocyclic ring enhances the quantum efficiencyand photochemical stability of the fluorophore. Compared to theirwidely used predecessor, “quina,” the new dyes offer up to 30-foldbrighter fluorescence, major changes in wavelength not just intensityupon Ca2+ binding, slightly lower affinities for Ca2+, slightly longerwavelengths of excitation, and considerably improved selectivity forCa2+ over other divalent cations. These properties, particularly thewavelength sensitivity to Ca2+, should make these dyes the preferredfluorescent indicators for many intracellular applications, especiallyin single cells, adherent cell layers, or bulk tissues. 这篇文章真的很牛，感兴趣的虫友看一下吧，是关于钙离子检测的。。。 http://d.namipan.com/d/895bf1094d3a3db93b3051fb6b519b0a8b6c40aef5a91500 [ Last edited by yunfeitian3525 on 2009-10-29 at 13:43 ] |
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2楼2009-10-29 11:03:47
liganghua
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yunfeitian3525
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