| 查看: 717 | 回复: 4 | |||
| 当前主题已经存档。 | |||
yunfeitian3525木虫 (正式写手)
|
[交流]
钱永健发的一篇引用次数超过13000的文章，大家参考一下
|
||
|
A New Generation of Ca2+ Indicatorsw ith Greatly Improved Fluorescence Properties* Grzegorz Grynkiewicz$, Martin Poenie, and Roger Y. TsienB From the Department of Physiology-Anatomy, Uniuersitoyf California, Berkeley, California9 4720 A new family of highly fluorescent indicators has been synthesized forbiochemical studies of the physiological role of cytosolic free Ca2+.The compounds combine an 8-coordinate tetracarboxylate chelating sitewith stilbene chromophores. Incorporation of the ethylenic linkage ofthe stilbene into a heterocyclic ring enhances the quantum efficiencyand photochemical stability of the fluorophore. Compared to theirwidely used predecessor, “quina,” the new dyes offer up to 30-foldbrighter fluorescence, major changes in wavelength not just intensityupon Ca2+ binding, slightly lower affinities for Ca2+, slightly longerwavelengths of excitation, and considerably improved selectivity forCa2+ over other divalent cations. These properties, particularly thewavelength sensitivity to Ca2+, should make these dyes the preferredfluorescent indicators for many intracellular applications, especiallyin single cells, adherent cell layers, or bulk tissues. 这篇文章真的很牛，感兴趣的虫友看一下吧，是关于钙离子检测的。。。 http://d.namipan.com/d/895bf1094d3a3db93b3051fb6b519b0a8b6c40aef5a91500 [ Last edited by yunfeitian3525 on 2009-10-29 at 13:43 ] |
回复此楼» 猜你喜欢
澳大利亚 Murdoch University 全奖博士招生（3个名额）地质化工冶金领域
已经有60人回复
-
我的国基提前知道中了，可是同事的操作让我实在接受不了，怎么会有这样的人
已经有15人回复
-
欢迎发来filecode的Mz6后的代码验证其规律
已经有29人回复
-
关于Filecode分析方法
已经有12人回复
-
咱们一起用铁证分析2026国家社科基金中标与否
已经有11人回复
-
小木虫上这么多卖论文的，真有人买论文么？感觉没必要啊
已经有10人回复
-
阿姨
已经有4人回复
-
一作与独作在应聘高校教师时区别大吗
已经有4人回复
-
静等基金结果
已经有21人回复
-
FileCode能看出啥？
已经有28人回复
2楼2009-10-29 11:03:47
liganghua
铁杆木虫 (著名写手)
露天使
- 应助: 2 (幼儿园)
- 金币: 7036.5
- 散金: 6
- 红花: 4
- 帖子: 1045
- 在线: 157.8小时
- 虫号: 825184
- 注册: 2009-08-10
- 专业: 农业昆虫学
3楼2009-10-29 11:17:08
yunfeitian3525
木虫 (正式写手)
- 应助: 0 (幼儿园)
- 金币: 1195.2
- 帖子: 359
- 在线: 129.9小时
- 虫号: 790654
- 注册: 2009-06-09
- 专业: 色谱分析
4楼2009-10-29 12:00:31
谢谢分享 |
5楼2009-10-30 08:42:08
