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lijietaren

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[交流] 质粒pUZ8002的图谱和大小

请问谁能提供质粒pUZ8002的图谱和大小?
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yzhang1986

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lijietaren(金币+2,VIP+0): 10-22 21:57
lijietaren(金币+1,VIP+0):谢谢 11-9 08:27
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2楼2009-10-22 18:28:26
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gyesang

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lijietaren(金币+15,VIP+0):谢谢 10-22 21:57
lijietaren(金币+2,VIP+0):谢谢 11-9 08:28
pUZ8002  RK2 derivative with defective oriT (aph)
RK2 Genebank登录号为:BN000925.1
Plasmid pUZ8002 is a derivative of RK2 with a mutation in oriT. The efficiency of conjugal transfer of this plasmid was 1,000-fold less than that of RK2, but it is capable of mobilizing other plasmids efficiently.
Plasmid pUZ8002 (J. W. Wilson and D. H. Figurski, unpublished data) is a derivative of pUZ8 (Kmr Tcr )  that carries the oriT1 mutation of an RK2 oriT1 derivative. The oriT1 mutation severely reduces pUZ8 self-transfer by several orders of magnitude (103). Wild-type pUZ8 self-transfer is similar to that of RK2 because it uses the same conjugation system found on RK2. The oriT1 mutation reduces self-transfer but not mobilization. Since self-transfer is not eliminated, rare self-transfer events can be selected easily.

所以你根据RK2的信息可以推测出pUZ8002的信息,包括图谱和大小,根据RK2做一个图谱,还是比较容易的,然后pUZ8002的图谱就是RK2图谱里oriT突变而已,Genebank里写得很详细,那一段序列分别代表什么

希望提供的信息对你有用,花了我好长时间才找到的

[ Last edited by gyesang on 2009-10-22 at 19:47 ]
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3楼2009-10-22 19:43:17
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