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There are many differences between soil freshly spiked with organochlorine pesticides and contaminated site soil which was polluted by organochlorine pesticides for many years. First of all, DDT and HCHs in contaminated site soil might have become less available because bioavailability of DDT (Singh and Agarwal, 1992) and HCHs (Quintero et al., 2005) diminishes with time. Furthermore, soils from former organochlorine pesticides-manufacturing plant maybe impacted by more than one contaminantant. Co-contaminants can complicate biodegradation (Foght et al., 2001). They provide more readily utilized substrates for the micro flora can divert enzymatic activity from the contaminant of concern and they have specific or nonspecific toxic effects on the soil micro flora and affect the solubility or adsorption of the contaminant of concern (Foght et al., 2001). Therefore, soils freshly amended with HCHs and DDX may not be good predictors of the biodegradation of aged HCHs and DDX in contaminated site that have become less available.

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