Znn3bq.jpeg
±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 221  |  »Ø¸´: 2
µ±Ç°Ö÷ÌâÒѾ­´æµµ¡£

andy9380

½ð³æ (СÓÐÃûÆø)

[½»Á÷] ÄÜÁ¿×ªÒƹ«Ê½µÄ»ý·Ö

Förster resonance energy transferÖÐÓйػý·ÖÄDz¿·ÖµÄ¼ÆËã¸ÃÈçºÎ½øÐÐ?

» ²ÂÄãϲ»¶

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

apzhough

Ìú³æ (СÓÐÃûÆø)

·¹ý£¬²È²È£»¹àË®ÎÞ×£¡£¡
2Â¥2009-09-28 16:34:18
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

vjyong

ľ³æ (СÓÐÃûÆø)

¡ï
andy9380(½ð±Ò+1,VIP+0):ÐÁ¿à·Ö 9-30 10:23
ÒýÓûØÌû:
Originally posted by andy9380 at 2009-9-22 10:38:
Förster resonance energy transferÖÐÓйػý·ÖÄDz¿·ÖµÄ¼ÆËã¸ÃÈçºÎ½øÐÐ?


where fD is the normalized donor emission spectrum, and ¦ÅA is the acceptor molar extinction coefficient. ¦Ê2 =2/3 is often assumed. This value is obtained when both dyes are freely rotating and can be considered to be isotropically oriented during the excited state lifetime. If either dye is fixed or not free to rotate, then ¦Ê2 =2/3 will not be a valid assumption. In most cases, however, even modest reorientation of the dyes results in enough orientational averaging that ¦Ê2 = 2/3 does not result in a large error in the estimated energy transfer distance due to the sixth power dependence of R0 on ¦Ê2. Even when ¦Ê2 is quite different from 2/3 the error can be associated with a shift in R0 and thus determinations of changes in relative distance for a particular system are still valid. Fluorescent proteins do not reorient on a timescale that is faster than their fluorescence lifetime. In this case 0 ¡Ü ¦Ê2 ¡Ü 4.
3Â¥2009-09-28 19:56:01
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ andy9380 µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 264Çóµ÷¼Á +9 ÂóС¶£µ± 2026-04-07 9/450 2026-04-08 01:52 by Linzejun
[¿¼ÑÐ] 315Çóµ÷¼Á +17 СÑòСÑò_ 2026-04-02 18/900 2026-04-07 22:01 by lijunpoly
[¿¼ÑÐ] 0703×Ü·Ö331Çóµ÷¼Á +16 ZY-05 2026-04-04 20/1000 2026-04-07 18:00 by À¶ÔÆË¼Óê
[¿¼ÑÐ] 081700ѧ˶£¬323·Ö£¬Ò»Ö¾Ô¸Öйúº£Ñó´óѧÇóµ÷¼ÁѧУ +19 ÅûÐÇºÓ 2026-04-04 19/950 2026-04-07 15:00 by Éϰ¶¿ì¿ì
[¿¼ÑÐ] ѧ˶»¯Ñ§¹¤³ÌÓë¼¼Êõ£¬Ò»Ö¾Ô¸Öйúº£Ñó´óѧ320+Çóµ÷¼Á +9 ÅûÐÇºÓ 2026-04-02 9/450 2026-04-07 12:53 by ¾¡Ë´Ò¢1
[¿¼ÑÐ] 080500Çóµ÷¼Á +12 »ÆÓ 2026-04-06 12/600 2026-04-07 12:41 by upczlm1989
[ÂÛÎÄͶ¸å] Decision: Revise for Editor»¹»áËÍÉóÂð 100+3 CccccccccFD 2026-04-04 5/250 2026-04-07 10:58 by ±±¾©À³ÒðÈóÉ«
[¿¼ÑÐ] Èí¹¤Ñ§Ë¶299Çóµ÷¼Á +6 useryy 2026-04-07 6/300 2026-04-07 09:50 by vgtyfty
[¿¼ÑÐ] 277¹¤¿ÆÇóµ÷¼Á +10 1915668 2026-04-04 11/550 2026-04-06 23:53 by ÐÄæÂÒâ±±
[¿¼ÑÐ] ²ÄÁϵ÷¼Á +10 Ò»ÑùYWY 2026-04-06 10/500 2026-04-06 21:05 by lbsjt
[¿¼ÑÐ] Çóµ÷¼Á +7 ÕÅ.1 2026-04-05 7/350 2026-04-05 20:40 by à£à£à£0119
[¿¼ÑÐ] 085500»úеר˶³õÊÔ288Çóµ÷¼Á +3 GZJguo666- 2026-04-05 3/150 2026-04-05 18:06 by jkddd
[¿¼ÑÐ] 295Çóµ÷¼Á +4 AÄãºÃÑо¿Éú 2026-04-04 5/250 2026-04-04 22:46 by yu221
[¿¼ÑÐ] 282µç×ÓÐÅÏ¢0854ר˶µ÷¼Á +4 202451007219 2026-04-02 6/300 2026-04-04 21:55 by laoshidan
[¿¼ÑÐ] 321Çóµ÷¼Á +6 ÈÏÕæÇóÉÏѧ 2026-04-03 6/300 2026-04-04 19:51 by dongzh2009
[¿¼ÑÐ] 22408£¬264Çóµ÷¼Á +3 ywh729 2026-04-03 4/200 2026-04-04 11:04 by ywh729
[¿¼ÑÐ] ¿¼Ñе÷¼Á +3 Draa 2026-04-03 3/150 2026-04-03 17:37 by hgwz7468
[¿¼ÑÐ] 320Çóµ÷¼Á +3 ũҵ¹¤³ÌÓëÐÅÏ¢¼ 2026-04-03 3/150 2026-04-03 11:40 by ÍÁľ˶ʿÕÐÉú
[¿¼ÑÐ] Ò»Ö¾Ô¸°²»Õ´óѧ0817»¯Ñ§¹¤³ÌÓë¼¼Êõ£¬Çóµ÷¼Á +14 ÎÒ²»ÊÇÖ»Òò 2026-04-02 15/750 2026-04-03 09:49 by À¶ÔÆË¼Óê
[¿¼ÑÐ] Ò»Ö¾Ô¸346ÉϺ£´óѧÉúÎïѧ +3 ÉϺ£´óѧ346µ÷¼Á 2026-04-01 3/150 2026-04-02 08:36 by w³æ³æ123
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û