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andy9380金虫 (小有名气)
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能量转移公式的积分
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| Förster resonance energy transfer中有关积分那部分的计算该如何进行? |
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2楼2009-09-28 16:34:18
vjyong
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andy9380(金币+1,VIP+0):辛苦分 9-30 10:23
andy9380(金币+1,VIP+0):辛苦分 9-30 10:23
 where fD is the normalized donor emission spectrum, and εA is the acceptor molar extinction coefficient. κ2 =2/3 is often assumed. This value is obtained when both dyes are freely rotating and can be considered to be isotropically oriented during the excited state lifetime. If either dye is fixed or not free to rotate, then κ2 =2/3 will not be a valid assumption. In most cases, however, even modest reorientation of the dyes results in enough orientational averaging that κ2 = 2/3 does not result in a large error in the estimated energy transfer distance due to the sixth power dependence of R0 on κ2. Even when κ2 is quite different from 2/3 the error can be associated with a shift in R0 and thus determinations of changes in relative distance for a particular system are still valid. Fluorescent proteins do not reorient on a timescale that is faster than their fluorescence lifetime. In this case 0 ≤ κ2 ≤ 4. |
3楼2009-09-28 19:56:01