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liangpeng1985铁杆木虫 (正式写手)
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【求助】【求助】KCL-TRIS怎么配制？
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0.6m KCL-TRIS怎么配制？ [ Last edited by liangpeng1985 on 2009-8-4 at 11:21 ] |
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liangpeng1985(金币+5,VIP+0): 8-4 16:23
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参考一下以下内容 Following hypotonie rupture of the cells with glass-distilled water and dilution (1:1) with 300 miviKC1-100 min Tris buffer (double strength KCl-Tris), the microsomal fractions from both the sensitive and the drug-resistant cells were obtained by differential centrifugation in KCl-Tris, as previously described ( 17). The mitochon-drial fractions were isolated by centrifugation in 0.25 Msucrose-25 mM Tris, pH 7.4, containing 1 mM EDTA and 1% BSA, followed by washing three times in KCl-Tris buffer, as previously described (18). The purified mitochondria and microsomes were suspended in KCl- Tris buffer and diluted appropriately following the determination of the protein content. |
2楼2009-08-04 13:59:13