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三磷酸腺苷

铁杆木虫 (职业作家)

[资源] 升级了，分享资源－常见细胞系和培养条件

漫长地从各种奇奇怪怪的虫终于升级到木虫了……
纪念一下，分享点资料

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Cell Line: 1G1
Cell Type: mouse hybridoma (anti-chicken dorsal retina cytoplasmic membrane s, E12)
DSMZ No: ACC 424
Origin: cell line was established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse
immunized with enriched retinal cell membranes; described as secreting a mouse monoclonal
antibody
(IgM) reacting with the chicken dorsal retina cytoplasmic membranes (E12) and binding outer
photoreceptor elements of adult vertebrate re tina (inclusive chick, mouse, rat)
References: Schlosshauer et al., Eur. J. Cell Biol. 73: 150-157 (1997)
Depositor: Dr. B. Schlosshauer, Natural and Medical Science Institute (NMI ), Reutlingen, Germany
DSMZ Cell Culture Data:
Morphology: spheroidal cells growing singly in suspension, or occasionally loosely adherent
Medium: 90% RPMI 1640 + 10% FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the firs t
1-2 weeks post-thawing)
Subculture: maintain at ca. 0.1-0.3 x 106
cells/ml; split saturated culture 1:3 to 1:10 every 2-5 days (some cells
attach to the surface of the culture flask, but can be detached by tapping the flask); seed out at ca.
0.3 x 106
cells/ml; a maximal density of 1.0 x 106
cells/ml should not be exceeded
Incubation: at 37 /C with 5% CO2
Doubling Time: doubling time of ca. 30 hours
Harvest: cell harvest of ca. 1.0 x 106
cells/ml
Storage: frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106
cells/ampoule
DSMZ Scientific Data:
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of mouse IgM monoclonal antibody
Species: confirmed as mouse with IEF of AST, LDH, NP
Cytogenetics: murine flat-moded hyper tetraploid karyotype; 94-102<4n>; no centric fusion markers present
Viruses: ELISA: reverse transcriptase negative

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