版块导航: 正在加载中...

登录注册

应《网络安全法》要求，自2017年10月1日起，未进行实名认证将不得使用互联网跟帖服务。为保障您的帐号能够正常使用，请尽快对帐号进行手机号验证，感谢您的理解与支持！

艾德科技（北京）有限公司

http://www.ivdshow.cn

表观遗传学（m6A RNA甲基化、RNA甲基化、DNA甲基化、DNA羟甲基、DNA甲酰基、DNA羧基），分子生物学，免疫生物学（一抗、二抗），细胞生物学，实验仪器学（核酸提起仪、超微量分光光度计）等等产品与技术服务

北京市昌平区中关村生命科学园东60米艾德园

联系人: Peter Chen / 邮箱: 1951545998@qq.com / QQ: 1513545070 / 电话: 0313-5935521 / 手机: 18911529660

关注收听

返回列表

【有奖交流】积极回复本帖子，参与交流，就有机会分得作者 ADTechnology 的 89 个金币，回帖就立即获得 1 个金币，每人有 1 次机会

ADTechnology

捐助贵宾 (著名写手)

表观遗传学专业供应商

应助: 12 (小学生)
金币: 12295.7
散金: 1743
红花: 1
帖子: 2534
在线: 45.9小时
虫号: 1787705
注册: 2012-04-30
专业: 人类遗传学

[商家供应] m6A RNA甲基化文库构建试剂盒【测序版】-（A-P-9016）-利器在手,实验无忧

本商家没有参加优惠活动，一起邀请他来参加吧我的代金券

代金券0元

我要兑换代金券

限量 x 张
满 x 可使用；有效期 x 天；过期可退

内容

EpiNext m6A RNA甲基化文库构建试剂盒(测序版) 货号：A-P-9016

EpiNext CUT&RUN RNA m6A-Seq Kit

背景资料：N6-methyladenosine (m6A) is the most common and abundant modification on RNA molecules present in eukaryotes. Recently, DNA m6A is also identified in multicellular eukaryotes including Caenorhabditis elegans and Drosophila melanogaster, and furthermore identified in higher eukaryotes including plants, mouse and human cells. m6A plays crucial roles in regulating DNA replication, DNA damage, RNA splicing, transposition, transcription, and cellular defense. In human cells, the m6A modification is probably catalyzed by a methyltransferase complex METTL3/METTL14 and removed by the α-ketoglutarate (α-KG)- and Fe2+-dependent dioxygenases such as FTO, ALKBH5 and TET-like enzymes. It was shown that METTL3 and α-KG /Fe2+-dependent dioxygenases play important roles in many biological processes, ranging from development and metabolism to fertility. The dynamic and reversible chemical m6A modification on DNA /RNA may also serve as a novel epigenetic marker of profound biological significance. Down-regulation of m6A modification was first characterized in human cancer cells and tissues, relative to their normal controls. m6A is found to be the most regulated DNA modification in cancers. In addition to the regulation in cancer cells, relative to the primary cell/tissues which contain quite low DNA m6A (<0.001%), a hundreds-fold increase of m6A modification was found for in vitro cultured human cells (0.03%-0.22%)。

产品描述：This kit is designed for measuring total m6A demethylase activity/inhibition. In an assay with this kit, the unique m6A substrate is stably coated on the strip wells. Active m6A demethylases bind to and demethylate m6A contained in the substrate. The un-demethylated m6A in the substrate can be recognized with a high affinity m6A antibody and the immuno-signal is enhanced with enhancer solution. The ratio or amount of un-demethylated m6A, which is inversely proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction。

产品特点：

1·High enrichment: Use RNA cleavage enzyme mix to simultaneously fragment RNA and cleave/remove any RNA sequences at both ends of the target m6A -containing sequences without affecting RNA regions occupied by the antibody. Short RNA fragments are generated only bound with anti- m6A antibody. True target m6A-enriched regions can, therefore, be reliably identified and high-resolution mapping achieved；

2·Low Input: Unbound RNA cleavage and immunocapture are processed in the same single-tube, which enables the maximal protection of the target m6A-containing regions and the minimized sample loss, allowing the input RNA to be as low as 500 ng；

3.Minimal Background: Cleavage of unbound RNA sequences in the two ends of the target m6A-containing sequences enables the minimized MeRIP/sequencing background, allowing data analysis with <10 million reads.
4.Fast, streamlined procedure: The procedure from RNA to library cDNA is less than 6 hours with <1 h of hands-on time；

5.Highly convenient: The kit contains all required components for each step of the CUT&RUN RNA m6A -Seq, which are sufficient for both m6A-containing RNA sequence capture and captured cDNA library preparation, thereby allowing CUT&RUN RNA m6A –Seq to be the most convenient with reliable and consistent results。
更多请参考:产品详情