| 查看: 433 | 回复: 20 | ||
| 【有奖交流】积极回复本帖子，参与交流，就有机会分得作者 ADTechnology 的 89 个金币 ，回帖就立即获得 1 个金币，每人有 1 次机会 | ||
ADTechnology
|
[商家供应]
m6A RNA甲基化文库构建试剂盒【测序版】-（A-P-9016）-利器在手,实验无忧
|
|
 EpiNext m6A RNA甲基化文库构建试剂盒(测序版) 货号：A-P-9016EpiNext CUT&RUN RNA m6A-Seq Kit 背景资料：N6-methyladenosine (m6A) is the most common and abundant modification on RNA molecules present in eukaryotes. Recently, DNA m6A is also identified in multicellular eukaryotes including Caenorhabditis elegans and Drosophila melanogaster, and furthermore identified in higher eukaryotes including plants, mouse and human cells. m6A plays crucial roles in regulating DNA replication, DNA damage, RNA splicing, transposition, transcription, and cellular defense. In human cells, the m6A modification is probably catalyzed by a methyltransferase complex METTL3/METTL14 and removed by the α-ketoglutarate (α-KG)- and Fe2+-dependent dioxygenases such as FTO, ALKBH5 and TET-like enzymes. It was shown that METTL3 and α-KG /Fe2+-dependent dioxygenases play important roles in many biological processes, ranging from development and metabolism to fertility. The dynamic and reversible chemical m6A modification on DNA /RNA may also serve as a novel epigenetic marker of profound biological significance. Down-regulation of m6A modification was first characterized in human cancer cells and tissues, relative to their normal controls. m6A is found to be the most regulated DNA modification in cancers. In addition to the regulation in cancer cells, relative to the primary cell/tissues which contain quite low DNA m6A (<0.001%), a hundreds-fold increase of m6A modification was found for in vitro cultured human cells (0.03%-0.22%)。 产品描述：This kit is designed for measuring total m6A demethylase activity/inhibition. In an assay with this kit, the unique m6A substrate is stably coated on the strip wells. Active m6A demethylases bind to and demethylate m6A contained in the substrate. The un-demethylated m6A in the substrate can be recognized with a high affinity m6A antibody and the immuno-signal is enhanced with enhancer solution. The ratio or amount of un-demethylated m6A, which is inversely proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction。 产品特点： 1·High enrichment: Use RNA cleavage enzyme mix to simultaneously fragment RNA and cleave/remove any RNA sequences at both ends of the target m6A -containing sequences without affecting RNA regions occupied by the antibody. Short RNA fragments are generated only bound with anti- m6A antibody. True target m6A-enriched regions can, therefore, be reliably identified and high-resolution mapping achieved； 2·Low Input: Unbound RNA cleavage and immunocapture are processed in the same single-tube, which enables the maximal protection of the target m6A-containing regions and the minimized sample loss, allowing the input RNA to be as low as 500 ng； 3.Minimal Background: Cleavage of unbound RNA sequences in the two ends of the target m6A-containing sequences enables the minimized MeRIP/sequencing background, allowing data analysis with <10 million reads. 4.Fast, streamlined procedure: The procedure from RNA to library cDNA is less than 6 hours with <1 h of hands-on time； 5.Highly convenient: The kit contains all required components for each step of the CUT&RUN RNA m6A -Seq, which are sufficient for both m6A-containing RNA sequence capture and captured cDNA library preparation, thereby allowing CUT&RUN RNA m6A –Seq to be the most convenient with reliable and consistent results。 更多请参考:产品详情  p-9016-03.png |
回复此楼» 本帖附件资源列表
-
欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：xiaomuchong@tal.com - 附件 1 : A-P-9016-EpiNext_m6A甲基化文库构建试剂盒(测序版).pdf
2020-06-02 18:13:52, 758.46 K
» 猜你喜欢
271材料工程求调剂
已经有5人回复
-
281求调剂（0805）
已经有16人回复
-
304求调剂
已经有6人回复
-
材料工程专硕调剂
已经有6人回复
-
一志愿天大材料与化工（085600）总分338
已经有4人回复
-
085700资源与环境308求调剂
已经有3人回复
-
求材料调剂
已经有8人回复
-
294求调剂材料与化工专硕
已经有5人回复
-
一志愿华中科技大学，080502，354分求调剂
已经有4人回复
-
一志愿吉林大学材料学硕321求调剂
已经有6人回复
» 抢金币啦！回帖就可以得到:
-
持续创业者，A8离异男征婚，事业遇到瓶颈，寻找创业生活伴侣
+1/485
-
海南大学化工院生物质定向转化课题组招收博士/硕士，2026年9月入学
+1/85
-
湘潭大学化工学院国家级人才团队2026年博士研究生招生
+1/64
-
青岛大学泰山学者课题组招2026年申请考核制博士
+1/33
-
重庆文理学院化学与环境工程学院 2026 届硕士研究生调剂通知
+1/16
-
学术/专业硕士调剂——化学、资源与环境
+1/15
-
国家级领军人才团队高分子材料方向2026年博士研究生招生
+1/15
-
Call for paper: [Fermentation – IF 3.3]
+2/14
-
【2026博士招生/博后招聘】北京航空航天大学潘彪课题组——AI芯片设计方向
+1/11
-
南京医科大学-膜蛋白结构与功能课题组-招收2026级博士研究生
+1/11
-
上海科技大学物质学院招收有机化学方向2026级工程博士
+1/7
-
教育部长江学者和创新团队发展计划”入选团队招收 材料，化学与化工博士研究生
+1/7
-
青岛科技大学可持续高分子团队 考研招生
+1/7
-
上海师范大学化学与材料科学学院任新意副研究员招收调剂学生3-4名（有机化学专业）
+1/5
-
中国科学院过程工程研究所助理研究员（正式职工）和博士后招聘启事
+1/5
-
课题组招收环境及相关专业调剂硕士研究生（欢迎优秀学生加入）
+1/2
-
125603工业工程与管理调剂
+1/2
-
085404 270求调剂，b区求导师收留接受跨专业
+1/1
-
中国科学院地球化学研究所招科研助理
+1/1
-
【2026年春季补录】厦门大学田中群院士/易骏教授纳米光子学团队招收2026年入学博士生
+1/1
2楼2020-06-02 18:14:16
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
 |
3楼2020-06-02 18:19:14
ADTechnology 
捐助贵宾 (著名写手)
表观遗传学专业供应商
- 应助: 12 (小学生)
- 金币: 12092.7
- 散金: 1762
- 红花: 1
- 帖子: 2608
- 在线: 49.1小时
- 虫号: 1787705
- 注册: 2012-04-30
- 专业: 人类遗传学
表观遗传学（m6A RNA甲基化、RNA甲基化、DNA甲基化、DNA羟甲基、DNA甲酰基、DNA羧基），分子生物学，免疫生物学（一抗、二抗），细胞生物学，实验仪器学（核酸提起仪、超微量分光光度计）等等产品与技术服务
北京市昌平区中关村生命科学园东60米艾德园
联系人: Peter Chen / 邮箱: 1951545998@qq.com / QQ: 1513545070 / 电话: 0313-5935521 / 手机: 18911529660
4楼2020-06-02 18:34:39
5楼2020-06-03 11:25:36
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
|
|
6楼2020-06-03 11:34:09
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
|
|
7楼2020-06-03 13:42:31
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
|
|
8楼2020-06-03 16:54:00
|
|
9楼2020-06-03 18:28:36
10楼2020-06-05 09:57:50
