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植物细胞凋亡检测
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| 各位好！我目前在做植物细胞凋亡检测，如果只做电镜观察和DNA LADDER，能说明细胞发生凋亡吗？证据充分吗？而且就目前的实验结果来看，电镜和DNA LADDER做的效果都不是很好，不知有没有做过这方面实验的同仁，恳请指点一二，谢谢！ |
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4楼2009-07-04 16:24:59
phoebecheung
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2楼2009-07-03 14:05:59
3楼2009-07-03 21:52:49
HarveyWang
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给你推荐你们专业的一篇文章，应该很有用的 Crucial role of antioxidant proteins and hydrolytic enzymes in pathogenicity of Penicillium expansum: analysis based on proteomics approach. Penicillium expansum, a widespread filamentous fungus, is a major causative agent of fruit decay and may lead to the production of mycotoxin that causes harmful effects on human health. In this study, we compared the cellular and extracellular proteomes of P. expansum in the absence and presence of borate, which affects the virulence of the fungal pathogen. The differentially expressed proteins were identified using ESI-Q-TOF-MS/MS. Several proteins related to stress response (glutathione S-transferase, catalase, and heat shock protein 60) and basic metabolism (glyceraldehyde-3-phosphate dehydrogenase, dihydroxy-acid dehydratase, and arginase) were identified in the cellular proteome. Catalase and glutathione S-transferase, the two antioxidant enzymes, exhibited reduced levels of expression upon exposure to borate. Because catalase and glutathione S-transferase are related to oxidative stress response, we further investigated the reactive oxygen species (ROS) levels and oxidative protein carbonylation (damaged proteins) in P. expansum. Higher amounts of ROS and carbonylated proteins were observed after borate treatment, indicating that catalase and glutathione S-transferase are important in scavenging ROS and protecting cellular proteins from oxidative damage. Additionally to find secretory proteins that contribute to the virulence, we studied the extracellular proteome of P. expansum under stress condition with reduced virulence. The expression of three protein spots were repressed in the presence of borate and identified as the same hydrolytic enzyme, polygalacturonase. 作 者： Qin,GTian,SChan,ZLi,B 刊 名： Molecular & Cellular Proteomics 年，卷(期)： 2007vol.6(no.3) |
5楼2009-07-05 03:03:48