| 查看: 388 | 回复: 5 | |||
| 当前主题已经存档。 | |||
[交流]
植物细胞凋亡检测
|
|||
| 各位好！我目前在做植物细胞凋亡检测，如果只做电镜观察和DNA LADDER，能说明细胞发生凋亡吗？证据充分吗？而且就目前的实验结果来看，电镜和DNA LADDER做的效果都不是很好，不知有没有做过这方面实验的同仁，恳请指点一二，谢谢！ |
回复此楼» 猜你喜欢
生物学学硕求调剂
已经有10人回复
-
上海电力大学材料防护与新材料重点实验室招收调剂研究生（材料、化学、电化学，环境）
已经有4人回复
-
材料学求调剂
已经有6人回复
-
303求调剂
已经有5人回复
-
一志愿武理085500机械专业总分300求调剂
已经有7人回复
-
考研调剂
已经有4人回复
-
281求调剂
已经有4人回复
-
0805 316求调剂
已经有6人回复
-
085601求调剂总分293英一数二
已经有3人回复
-
08工学调剂
已经有17人回复
phoebecheung
金虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 19.7
- 散金: 54
- 帖子: 105
- 在线: 252.9小时
- 虫号: 490165
- 注册: 2008-01-03
- 专业: 植物生理与生化
2楼2009-07-03 14:05:59
3楼2009-07-03 21:52:49
4楼2009-07-04 16:24:59
HarveyWang
捐助贵宾 (知名作家)
海外行者
- BioEPI: 14
- 应助: 80 (初中生)
- 金币: 2277.2
- 散金: 24
- 红花: 65
- 帖子: 6880
- 在线: 946小时
- 虫号: 528557
- 注册: 2008-03-19
- 性别: GG
- 专业: 微生物生理与生物化学
★
小木虫(金币+0.5):给个红包，谢谢回帖交流
小木虫(金币+0.5):给个红包，谢谢回帖交流
给你推荐你们专业的一篇文章，应该很有用的 Crucial role of antioxidant proteins and hydrolytic enzymes in pathogenicity of Penicillium expansum: analysis based on proteomics approach. Penicillium expansum, a widespread filamentous fungus, is a major causative agent of fruit decay and may lead to the production of mycotoxin that causes harmful effects on human health. In this study, we compared the cellular and extracellular proteomes of P. expansum in the absence and presence of borate, which affects the virulence of the fungal pathogen. The differentially expressed proteins were identified using ESI-Q-TOF-MS/MS. Several proteins related to stress response (glutathione S-transferase, catalase, and heat shock protein 60) and basic metabolism (glyceraldehyde-3-phosphate dehydrogenase, dihydroxy-acid dehydratase, and arginase) were identified in the cellular proteome. Catalase and glutathione S-transferase, the two antioxidant enzymes, exhibited reduced levels of expression upon exposure to borate. Because catalase and glutathione S-transferase are related to oxidative stress response, we further investigated the reactive oxygen species (ROS) levels and oxidative protein carbonylation (damaged proteins) in P. expansum. Higher amounts of ROS and carbonylated proteins were observed after borate treatment, indicating that catalase and glutathione S-transferase are important in scavenging ROS and protecting cellular proteins from oxidative damage. Additionally to find secretory proteins that contribute to the virulence, we studied the extracellular proteome of P. expansum under stress condition with reduced virulence. The expression of three protein spots were repressed in the presence of borate and identified as the same hydrolytic enzyme, polygalacturonase. 作 者： Qin,GTian,SChan,ZLi,B 刊 名： Molecular & Cellular Proteomics 年，卷(期)： 2007vol.6(no.3) |
5楼2009-07-05 03:03:48
6楼2009-07-05 23:09:13
