±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 6608  |  »Ø¸´: 2
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

È˼䷼·Æ

½û³æ (ÕýʽдÊÖ)

±¾ÌûÄÚÈݱ»ÆÁ±Î

ÒÑÔÄ   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÄãÊÇ·È˼×

Í­³æ (СÓÐÃûÆø)

ÎÒÃÇʵÑéÊÒÓÃRFect sRNA Transfection Reagent£¬Ï¸°ûÃܶÈÔÚ45%×óÓÒ ,ÉèÖÃsiRNAÖÕŨ¶È10nM,30nM,50nM,100nMËĸöÌݶȣ¬Ã¿¸öÌݶÈ×îºÃ×ö2¸ö¸´¿×£¬Ò»°ã×öתȾǰһÌìÆÌ°å£¬ÓÃÀ´Ï¡ÊÍsiRNAºÍÏ¡ÊÍתȾÊÔ¼ÁµÄÅàÑø»ù±ØÐëÊÇÎÞѪÇåÅàÑø»ù£¬ÒòΪѪÇåµÄ´æÔÚ»á¸ÉÈÅsiRNAÓëתȾÊÔ¼ÁµÄ»ìºÏ£¬²¢ÇÒÓ°ÏìתȾЧÂÊ¡£×ªÈ¾ºó48hÊÕϸ°û×öqPCR»ùÒòˮƽ¼ì²â»òÕßתȾºó72hÊÕϸ°ûÌáµ°°××öwestern Blottingµ°°×ˮƽ¼ì²â£¬Ò»°ãÇé¿öÉèÖÃÒ»¸ö¿Õ°××飬һ¸ö¶ÔÕÕ×é
3Â¥2019-11-13 11:50:22
ÒÑÔÄ   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 3 ¸ö»Ø´ð

Explor

гæ (ÕýʽдÊÖ)

¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
ÒÔRFectСºËËáתȾÊÔ¼ÁΪÀý
ÊÊÓÃÓÚ24¿×ÅàÑø°åµÄתȾʵÑ飬ÆäËû¹æ¸ñµÄÅàÑø°åµÄÓÃÁ¿²ÎÕÕÏÂÃæ±í¸ñ£¬±í¸ñ¸ø³öµÄÊÇÿ¿×µÄÓÃÁ¿ÓëÌå»ý¡£
A. ϸ°û½ÓÖÖ£º×ªÈ¾Ç°Ò»Ìì½ÓÖÖϸ°û£¬Ã¿¿×500 ¦ÌlÅàÑø»ù£¨²»¿É¼Ó¿¹ÉúËØ£©£¬Ê¹Ï¸°ûÔÚתȾʱÃܶÈÔÚ30-50%¡£
B. siRNA-RFect»ìºÏÎï×¼±¸£º
1.6pmol siRNA ÓÃ50¦ÌlÎÞѪÇåÅàÑø»ùÏ¡ÊÍ¡£
2.2¦Ìl RFectÓÃ50¦ÌlÎÞѪÇåÅàÑø»ùÏ¡ÊÍ¡£ÇáÇá»ìÔÈ£¬ÊÒηõÓý5min¡£×¢Ò⣺ȷ±£ÔÚ25 minÄÚÖ´ÐеÚÈý²½²Ù×÷£¬²»Òª¹ýÓÚÑÓ³Ù¡£
3.·õÓý5minºó£¬½«siRNA Ï¡ÊÍÒºÓëRFect Ï¡ÊÍÒº»ìºÏ£¨×ÜÌå»ý100¦Ìl£©¡£ÇáÇá»ìÔÈ£¬ÊÒηõÓý20 min¡£
C. ½«»ìºÏÎï¼Óµ½ÅàÑøµÄϸ°ûÄÚ£¨ÍêÈ«ÅàÑø»ùÅàÑø£©£º
1.½«100¦Ìl»ìºÏÎï¼ÓÈëÅàÑø¿×ÄÚ£¬ÅàÑø¿×ÄÚº¬ÓÐ0.5mlÅàÑøµÄϸ°û¡£ÇáÇá»Î¶¯ÅàÑø°å£¬»ìÔÈ¡£
2.37¡ãCÅàÑø18-72h£¬¼ì²â»ùÒòÒÖÖÆÐ§¹û¡£Èç¹ûÐèÒª£¬Ï¸°ûÅàÑø4-6hʱ¿ÉÒÔ¸ü»»ÅàÑø»ù£¬µ«²»ÊDZØÐë¡£·õÓýʱ¼äµÄ³¤¶Ì£¬È¡¾öÓÚϸ°ûÀàÐÍ¡¢
Ëù¸ÉÈÅ»ùÒò±¾Éí¼°·ÖÎö·½·¨¡£¿ÉÉèÖò»Í¬µÄ·õÓýʱ¼ä½øÐÐʵÑéÒÔÈ·¶¨×î¼Ñ·õÓýʱ¼ä¡£
תȾʵÑéÒªµã£º
תȾ¹ý³Ì²»¿ÉÌí¼Ó¿¹ÉúËØ£¬·ñÔò»áµ¼ÖÂϸ°ûËÀÍö£»
Ê×´ÎʵÑésiRNAµÄÓÃÁ¿ÉèÖÃÔÚÖÕŨ¶È10nM¡¢30nM¡¢50nM¡¢100 nM½øÐÐÃþË÷ £¬ºóÐøÊµÑé¸ù¾ÝʵÑé½á¹ûÐ޸ġ£
תȾʵÑéÓÅ»¯: ΪÁËÌá¸ßתȾЧÂÊ£¬×îºÃ¶ÔתȾÌõ¼þ½øÐÐÓÅ»¯£¬ÌرðÊÇÊ×´ÎʹÓá£ÀýÈ磺24¿×ÅàÑø°å£¬µ÷Õû siRNAÓëRFectÊÔ¼ÁµÄÓÃÁ¿¡£
siRNA ÓÃÁ¿ÔÚ0.6-30 pmol(final concentration 1- 50 nM)Ö®¼äµ÷Õû£¬RFectÊÔ¼ÁÓÃÁ¿ÔÚ1.0 - 3.0¦ÌlÖ®¼äµ÷Õû¡£¿Í»§¿É°´ÕÕϰ¹ßÓÃÁ¿½øÐÐԤʵÑ飬ÈçʵÑé½á¹û²»ÀíÏ룬¿ÉÊʵ±µ÷Õû²¢ÃþË÷תȾÊÔ¼ÁÓÃÁ¿¡£
¸ü¶àÄÚÈÝ¿ÉÒÔµ½www.biodai.com.cn×Éѯ
¸öÐÔ»¯»ùÒòÑо¿¡¢¿ÆÑÐÊÔ¼Á¡¢Õï¶ÏÊÔ¼ÁºÍʵÑé·þÎñ
2Â¥2019-10-28 08:44:49
ÒÑÔÄ   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 320·Ö£¬²ÄÁÏÓ뻯¹¤×¨Òµ£¬Çóµ÷¼Á +4 Ò»¶¨Éϰ¶aaa 2026-03-27 4/200 2026-03-27 21:33 by ËÉ»¨¸×1201
[¿¼ÑÐ] 328Çóµ÷¼Á +6 àŵεĻù±¾¶¼ 2026-03-27 6/300 2026-03-27 16:49 by sanrepian
[¿¼ÑÐ] »¯Ñ§308·ÖÇóµ÷¼Á +8 ÄãºÃÃ÷ÌìÄãºÃ 2026-03-23 9/450 2026-03-27 14:01 by Ñî¹âÓÚÇàÔÆ
[¿¼ÑÐ] 305Çóµ÷¼Á +5 ÍÛ¬¿¨¿â 2026-03-26 5/250 2026-03-27 14:01 by laoshidan
[¿¼ÑÐ] Ò»Ö¾Ô¸»ª¶«Àí¹¤´óѧ081700£¬³õÊÔ·ÖÊý271 +6 kotoko_ik 2026-03-23 7/350 2026-03-27 12:29 by »ÝÖÝÅíÓÚêÌ
[¿¼ÑÐ] 085601 ²ÄÁϹ¤³Ì 313·Ö Çóµ÷¼Á +5 Ong3 2026-03-27 5/250 2026-03-27 12:24 by goldfish51
[¿¼ÑÐ] 275Çóµ÷¼Á +10 Micky11223 2026-03-25 12/600 2026-03-27 09:41 by caszguilin
[¿¼ÑÐ] 081200-11408-276ѧ˶Çóµ÷¼Á +3 ´Þwj 2026-03-26 3/150 2026-03-27 07:58 by chemisry
[¿¼ÑÐ] 081200-11408-276ѧ˶Çóµ÷¼Á +3 ´Þwj 2026-03-26 3/150 2026-03-26 19:57 by nihaoar
[¿¼ÑÐ] ×Ü·Ö322ÇóÉúÎïѧ/Éú»¯Óë·Ö×Ó/ÉúÎïÐÅϢѧÏà¹Øµ÷¼Á +5 ÐdzÁuu 2026-03-26 6/300 2026-03-26 19:02 by macy2011
[¿¼ÑÐ] ÉúÎï¼¼ÊõÓ빤³Ì +3 1294608413 2026-03-25 4/200 2026-03-25 18:02 by 1294608413
[¿¼ÑÐ] 0854µç×ÓÐÅÏ¢Çóµ÷¼Á 324 +4 Promise-jyl 2026-03-23 4/200 2026-03-25 11:36 by Sugarlight
[¿¼ÑÐ] Ò»Ö¾Ô¸¼ªÁÖ´óѧ²ÄÁÏÓ뻯¹¤303·ÖÇóµ÷¼Á +4 Ϊѧ666 2026-03-24 4/200 2026-03-25 11:27 by BruceLiu320
[¿¼ÑÐ] 292Çóµ÷¼Á +4 ¶ì¶ì¶ì¶î¶î¶î¶î¶ 2026-03-24 4/200 2026-03-24 16:41 by peike
[»ù½ðÉêÇë] Çë½ÌÏ´ó¼Ò 2026Äê¹ú¼Ò»ù½ðÉêÇëÊÇ˫äÉóÂ𣿠+3 lishucheng1 2026-03-22 5/250 2026-03-24 08:22 by gltch
[¿¼ÑÐ] 361Çóµ÷¼Á +3 Glack 2026-03-22 3/150 2026-03-23 22:03 by fuyu_
[¿¼ÑÐ] 070300£¬Ò»Ö¾Ô¸±±º½320Çóµ÷¼Á +3 Jerry0216 2026-03-22 5/250 2026-03-23 09:16 by ¡£¡£ÌÃÌÃ
[¿¼ÑÐ] Ò»Ö¾Ô¸±±¾©»¯¹¤´óѧ070300 ѧ˶336Çóµ÷¼Á +5 vvÃÔ 2026-03-21 8/400 2026-03-22 14:20 by ColorlessPI
[¿¼ÑÐ] 0805²ÄÁÏ320Çóµ÷¼Á +3 ÉÎïÓï 2026-03-20 3/150 2026-03-21 15:46 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] 085601µ÷¼Á 358·Ö +3 zzzzggh 2026-03-20 4/200 2026-03-21 10:21 by luoyongfeng
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û