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°®ÓëÓêÏÂ: ½ð±Ò+1 2019-06-13 21:58:03
С¼¦µ°¡«(fjtony163´ú·¢): ½ð±Ò+10, ´ú·¢ 2019-07-19 00:27:49
fjtony163: ·ÒëEPI+1, ´ú·¢ 2019-07-19 00:27:54
°®ÓëÓêÏÂ: ½ð±Ò+1 2019-06-13 21:58:03
С¼¦µ°¡«(fjtony163´ú·¢): ½ð±Ò+10, ´ú·¢ 2019-07-19 00:27:49
fjtony163: ·ÒëEPI+1, ´ú·¢ 2019-07-19 00:27:54
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Cell suspension was centrifuged at 4000rpm for 20 min and 10 ml of cell-free supernatant harvested during the stationary growth phase was extracted using the procesdure described previously [30] ϸ°ûÐüÒºÒÔ4000rpmÀëÐÄ20 min£¬È»ºóÀûÓÃÇ°ÃæÃèÊöµÄ¹¤ÒÕÌáÈ¡¾²Ö¹Éú³¤½×¶ÎÊÕ»ñµÄ10 mlÎÞϸ°ûÉÏÇåÒº[30]¡£ In brief, 10 ml of cell-free supernatant solution was extracted three times with an equal amount of chloroform. ¼ò¶øÑÔÖ®£¬È¡10mlÎÞϸ°ûÉÏÇåÒº£¬ÓõÈÁ¿ÂÈ·ÂÈý´ÎÝÍÈ¡¡£ The combined orgainc phases were washed with 2 mL of water and taken to dryness in the oven kept at 20. ½«»ìºÏºóµÄÓлúÏàÓÃ2ºÁÉýË®³åÏ´¸É¾»£¬·ÅÈ뿾Ïä¸ÉÔζȱ£³ÖÔÚ20¡æ¡£ The residue was redissolved in 2 mL of acetonitrile. ²ÐÔüÔÚ2ºÁÉýÒÒëæÖÐÖØÐÂÈܽ⡣ This culture solution was stored at freezing temperature until further use. ¸ÃÅàÑøÒºÀä¶³±£´æ£¬´ý½øÒ»²½Ê¹Óᣠ|
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