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爱与雨下: 金币+1 2019-06-13 21:58:03
小鸡蛋～(fjtony163代发): 金币+10, 代发 2019-07-19 00:27:49
fjtony163: 翻译EPI+1, 代发 2019-07-19 00:27:54

Cell suspension was centrifuged at 4000rpm for 20 min and 10 ml of cell-free supernatant harvested during the stationary growth phase was extracted using the procesdure described previously [30]
细胞悬液以4000rpm离心20 min，然后利用前面描述的工艺提取静止生长阶段收获的10 ml无细胞上清液[30]。

In brief, 10 ml of cell-free supernatant solution was extracted three times with an equal amount of chloroform.
简而言之，取10ml无细胞上清液，用等量氯仿三次萃取。

The combined orgainc phases were washed with 2 mL of water and taken to dryness in the oven kept at 20.
将混合后的有机相用2毫升水冲洗干净，放入烤箱干燥，温度保持在20℃。

The residue was redissolved in 2 mL of acetonitrile.
残渣在2毫升乙腈中重新溶解。

This culture solution was stored at freezing temperature until further use.
该培养液冷冻保存，待进一步使用。

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