±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 405  |  »Ø¸´: 3
µ±Ç°Ö÷ÌâÒѾ­´æµµ¡£

fjtcm8772

½ð³æ (СÓÐÃûÆø)

[½»Á÷] ÇóÖú·­ÒëÒ»¶ÎÕªÒª

The purpose of this study was to develop a method for the stereoselective analysis of thioridazine-2-sulfoxide (THD-2-SO) and thioridazine-5-sulfoxide (THD-5-SO) in culture medium and to study the biotransformation of rac-thioridazine (THD) by some endophytic fungi. The simultaneous resolution of THD-2-SO and THD-5-SO diastereoisomers was performed on a CHIRALPAK® AS column using a mobile phase of hexane:ethanol:methanol (92:6:2, v/v/v) + 0.5%diethylamine; UV detection was carried out at 262 nm. Diethyl ether was used as extractor solvent.The validated method was used to evaluate the biotransformation of THD by 12 endophytic fungi isolated from Tithonia diversifolia, Viguiera arenaria and Viguiera robusta.Among the 12 fungi evaluated, 4 of themdeserve prominence for presenting an evidenced stereoselective biotransformation potential: Phomopsis sp. (TD2) presented greatermono-2-sulfoxidation to the form(S)-(SE) (12.1%);Glomerella cingulata (VA1) presented
greater mono-5-sulfoxidation to the forms (S)-(SE) + (R)-(FE) (10.5%); Diaporthe phaseolorum (VR4) presented greater mono-2-sulfoxidation to the forms (S)-(SE) and (R)-(FE) (84.4% and 82.5%, respectively) and Aspergillus fumigatus (VR12) presented greater mono-2-sulfoxidation to
the forms (S)-(SE) and (R)-(SE) (31.5% and 34.4%, respectively).


Õæ¾úÃû´Ê¿ÉÒÔ²»·­Òë¡£¡£¡£¡£

» ²ÂÄãϲ»¶

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

liubencai

ľ³æÖ®Íõ (ÎÄ̳¾«Ó¢)

¡ï ¡ï ¡ï ¡ï ¡ï
fjtcm8772(½ð±Ò+5,VIP+0): 5-1 18:54
The purpose of this study was to develop a method for the stereoselective analysis of thioridazine-2-sulfoxide (THD-2-SO) and thioridazine-5-sulfoxide (THD-5-SO) in culture medium and to study the biotransformation of rac-thioridazine (THD) by some endophytic fungi.
±¾Ñо¿µÄÄ¿µÄÊÇÑо¿ÔÚÅàÑø½éÖÊÖм×ÁòßÕàº-2-ÑÇí¿ (THD-2-SO)ºÍ¼×ÁòßÕàº-5-ÑÇí¿ (THD-5-SO)µÄÁ¢ÌåÑ¡ÔñÐÔ·ÖÎö£¬ÒÔ¼°Í¨¹ýijЩÄÚÉúÕæ¾úÑо¿ÍâÏûÐý-¼×ÁòßÕàº(THD)µÄÉúÎïת»¯¡£ (¼×ÁòßÕàºÓÖ³ÆÁòÀû´ïàº)

The simultaneous resolution of THD-2-SO and THD-5-SO diastereoisomers was performed on a CHIRALPAK®
ÔÚCHIRALPAK®ÊÖÐÔÖùÉϽ«THD-2-SOºÍTHD-5-SOµÄ·Ç¶ÔÓ³Òì¹¹Ìåͬʱ·Ö±æ¿ª£»

AS column using a mobile phase of hexane:ethanol:methanol (92:6:2, v/v/v) +diethylamine;
Öù×ÓÓüºÍé:ÒÒ´¼:¼×´¼(92:6:2, v/v/v)+ 0.5%¶þÒÒ°·×÷ΪÁ÷¶¯Ïࣻ

UV detection was carried out at 262 nm.
ÔÚ262 nm½øÐÐ×ÏÍâ¼à²â¡£

Diethyl ether was used as extractor solvent.
ÒÒÃÑÓÃ×÷ÝÍÈ¡ÈܼÁ(ÁÜÏ´¼Á)¡£

The validated method was used to evaluate the biotransformation of THD by 12 endophytic fungi isolated from Tithonia diversifolia, Viguiera arenaria and Viguiera robusta.
¸ÃÓÐЧµÄ·½·¨ÓÃÓÚÆÀ¼ÛTHDµÄÉúÎïת»¯ÊÇͨ¹ý´ÓÖ×±ú¾Õ¡¢Viguiera arenariaºÍViguiera robusta·ÖÀë³öÀ´µÄ12ÖÖÄÚÉúÕæ¾ú½øÐеġ£

Among the 12 fungi evaluated, 4 of them deserve prominence for presenting an evidenced stereoselective biotransformation potential:
±»ÆÀ¼ÛµÄ12ÖÖÕæ¾úÖУ¬ÆäÖÐ4Öֵõ½Á˳ÊÏÖ³öÓÐÖ¤¾ÝµÄÁ¢ÌåÑ¡ÔñÉúÎïת»¯µÄDZÖÊ¡£

[ Last edited by liubencai on 2009-5-1 at 07:51 ]
¼á³Öµ½µ×
2Â¥2009-04-30 23:47:53
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

liubencai

ľ³æÖ®Íõ (ÎÄ̳¾«Ó¢)

¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
fjtcm8772(½ð±Ò+5,VIP+0): 5-1 18:54
fjtcm8772(½ð±Ò+5,VIP+0): 5-2 19:12
Phomopsis sp. (TD2) presented greater mono-2-sulfoxidation to the form (S)-(SE) (12.1%);
½ÏºÃµÄµ¥-2-»Ç»¯Ñõ»¯×÷ÓÃʹµÃÄâ¾¥µãùÊô(TD2)µÃµ½(S)-(SE)ÐÎʽ(12.1%)£»

Glomerella cingulata  presented greater mono-5-sulfoxidation to the forms (S)-(SE) + (R)-(FE) (10.5%);  
½ÏºÃµÄµ¥-5-»Ç»¯Ñõ»¯×÷ÓÃʹµÃΧС´Ô¿Ç(VA1)µÃµ½(S)-(SE) + (R)-(FE)ÐÎʽ(10.5%);

Diaporthe phaseolorum (VR4) presented greater mono-2-sulfoxidation to the forms (S)-(SE) and (R)-(FE) (84.4% and 82.5%, respectively) and Aspergillus fumigatus (VR12) presented greater mono-2-sulfoxidation to the forms (S)-(SE) and (R)-(SE) (31.5% and 34.4%, respectively).
½ÏºÃµÄ¼äµ¥-2-»Ç»¯Ñõ»¯×÷ÓÃʹµÃ×ù¿ÇÊôphaseolorum (VR4)µÃµ½(S)-(SE)ºÍ(R)-(FE)ÐÎʽ(·Ö±ðÊÇ84.4%ºÍ82.5%)ÒÔ¼°½ÏºÃµÄµ¥-2-»Ç»¯Ñõ»¯×÷ÓÃʹµÃÑÌÇúù(VR12)µÃµ½(S)-(SE)ºÍ(R)-(SE)ÐÎʽ(·Ö±ðÊÇ31.5%ºÍ34.4%)¡£
¼á³Öµ½µ×
3Â¥2009-05-01 08:49:28
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

zw0912

½ð³æ (ÕýʽдÊÖ)

ÎÞΪÔòÎÞ²»Îª

·¹ý²Î¹Ûһϣ¬¿´¿´¸÷λ¸ßÊÖµÄˮƽ
ÐÞÉí¡¢ÐÞÐÄ¡¢Ð޵¡¢ÐÞÖÇ
4Â¥2009-05-01 22:25:01
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ fjtcm8772 µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 0703µ÷¼Á +14 ʰ¾ÁÒ¼ 2026-04-04 16/800 2026-04-06 23:03 by chenzhimin
[¿¼ÑÐ] ²ÄÁϵ÷¼Á +14 СÁõͬѧ߹߹ 2026-04-06 15/750 2026-04-06 22:37 by qlm5820
[¿¼ÑÐ] 08600ÉúÎïÓëÒ½Ò©-327 +9 18755400796 2026-04-05 9/450 2026-04-06 22:35 by 52305043001
[¿¼ÑÐ] 297·Ö083200ÇóÖú +9 aekx 2026-04-05 9/450 2026-04-06 20:57 by flysky1234
[¿¼ÑÐ] 081700£¬311£¬Çóµ÷¼Á +16 ¶¬Ê®Èý 2026-04-04 17/850 2026-04-06 14:56 by ÉÐË®¸óÖ÷
[¿¼ÑÐ] ²ÄÁÏר˶322·Ö +10 ¹þ¹þ¹þºðºðºð¹þ 2026-04-04 10/500 2026-04-05 21:22 by ѧԱ8dgXkO
[¿¼ÑÐ] 301Çóµ÷¼Á +3 XYPLR 2026-04-05 4/200 2026-04-05 19:07 by XYPLR
[¿¼ÑÐ] ¹¤¿Æ08-»úеר˶-Çóµ÷¼Á +3 À×Å··ÉÌß 2026-04-02 3/150 2026-04-05 18:49 by À¶ÔÆË¼Óê
[¿¼ÑÐ] 358Çóµ÷¼Á +7 Çïgk 2026-04-04 7/350 2026-04-05 13:29 by huangmoli
[¿¼ÑÐ] Äܶ¯µ÷¼Á326ר˶ +4 wan112233 2026-04-04 4/200 2026-04-04 22:47 by yu221
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤306·ÖÕÒµ÷¼Á +23 ²×º£ÇáÖÛe 2026-04-02 27/1350 2026-04-04 21:52 by laoshidan
[¿¼ÑÐ] 081200-11408-276ѧ˶Çóµ÷¼Á +5 ´Þwj 2026-03-31 5/250 2026-04-04 19:45 by 1753564080
[¿¼ÑÐ] ±¾¿Æ211£¬×¨Òµ085404£¬293·ÖÇëÇóµ÷¼Á +5 Á«²Ë¾ÍÊÇź°É 2026-04-04 5/250 2026-04-04 14:08 by ÕâÊÇÒ»¸öÎÞÁĵÄê
[¿¼ÑÐ] 309Çóµ÷¼Á +6 ÁõÁõÁõ1231 2026-04-02 7/350 2026-04-04 13:41 by liucky
[¿¼ÑÐ] µ÷¼Á0855-288 +5 xÐܶþa 2026-04-03 5/250 2026-04-04 00:19 by Öí»á·É
[¿¼ÑÐ] Ò»Ö¾Ô¸ÖйúʯÓÍ´óѧ»¯Ñ§¹¤³Ì323·ÖÇóµ÷¼Á +4 »¯¹¤×¨Ë¶323·Ö 2026-04-03 6/300 2026-04-03 22:12 by dongzh2009
[¿¼ÑÐ] 081200-11408-276ѧ˶Çóµ÷¼Á +6 ´Þwj 2026-04-02 6/300 2026-04-03 10:19 by À¶ÔÆË¼Óê
[¿¼ÑÐ] 26¿¼Ñе÷¼Á +4 Wnz.20030617 2026-04-01 5/250 2026-04-02 16:11 by 1939136013¹·×³
[¿¼ÑÐ] 272Çóµ÷¼Á£¬½ÓÊÜ¿çרҵµ÷¼Á£¡ +4 ÏÐÓ㬠2026-03-31 4/200 2026-04-02 11:18 by guyan1000
[¿¼ÑÐ] 372Çóµ÷¼Á +3 jjÓ¿77 2026-04-02 3/150 2026-04-02 09:57 by olim
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û