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| 毛裂蜂斗菜根茎粉碎，过14目筛，用95%乙醇作提取溶媒，先以等倍量溶媒浸润24h，再收集5倍量溶媒渗漉，渗漉速度为0.4BV/h提取；收集提取液，减压浓缩至无醇味，冷却、滤液加蒸馏水稀释到浓度为0.8~1.9﹕1（药材重量﹕溶液体积），即为上柱样品液。将上柱样品液按0.6BV上样量通入装有树脂的色谱柱中，依次用60%、80%、95％乙醇洗脱，流速为2.5 BV/h。然后将80%乙醇洗脱液减压浓缩至干，可得蜂斗菜总内酯。 |
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2楼2009-04-17 17:15:33
jerry.lubo
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3楼2009-04-17 17:32:58
4楼2009-04-17 18:48:57
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qingfeng877680(金币+5,VIP+0):辛苦啦！代楼主酬谢你！ 6-2 19:05
qingfeng877680(金币+5,VIP+0):辛苦啦！代楼主酬谢你！ 6-2 19:05
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减压浓缩至无醇味，冷却、滤液加蒸馏水稀释到浓度为0.8~1.9﹕1（药材重量﹕溶液体积），即为上柱样品液。将上柱样品液按0.6BV上样量通入装有树脂的色谱柱中，依次用60%、80%、95％乙醇洗脱，流速为2.5 BV/h。 Condense it under reduced pressure till there is no smell of alcohol. Cool down and dilute it to the concentration of 0.8~1.9:1(Wt solute : Wt solvent) with distilled water.That is sample for chromatography. Inject 0.8BV of sample to the column which is coated with resin. Elute the sample with 60%、80% and 95％ ethonal in series. The flow rate is 2.5 BV/h. |
5楼2009-04-19 05:50:33
zw0912
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qingfeng877680(金币+5,VIP+0):代楼主酬谢你，辛苦了！ 6-2 19:06
qingfeng877680(金币+5,VIP+0):代楼主酬谢你，辛苦了！ 6-2 19:06
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Mao cracked crush butterbur root vegetables, 14 mesh, with 95% ethanol as extraction solvent, the volume of first solvent, such as infiltration times 24h, and then 5 times the volume of solvent collected shenlu dialysis, dialysis flow rate of 0.4BV / h extraction; collection extract, decompression enrichment to non-alcohol taste, cooling, distilled water and diluted filtrate added to a concentration of 0.8 ~ 1.9:1 (herbal weight: solution volume), which is on the column sample liquid. Liquid samples will be on the column sample by 0.6BV with access to the volume of resin in the column, followed by 60%, 80%, 95% ethanol elution, flow rate of 2.5 BV / h. 80% ethanol and then vacuum concentrated eluate to dry dishes available butterbur total lactones 试着翻译一下，请多指正 |
6楼2009-04-21 08:48:41