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“transductional crosses ”该怎么理解？【有效期至2009年4月15日】
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By four transductional crosses as indicated in Figure 5, the mutations described above were combined in a single strain D-60 defective in threonine(苏氨酸)-degrading emzymes. 这里的“transductional crosses ”该怎么理解？ |
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4楼2009-04-13 10:29:02
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zhtt1983(金币+2,VIP+0): 4-20 12:23
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By four transductional crosses as indicated in Figure 5, the mutations described above were combined in a single strain D-60 defective in threonine(苏氨酸)-degrading emzymes. 正如图5 所示，通过四次转导杂交过程，上文所提到的突变，在苏氨酸降解酶中，转化成一种单一品系的D-60缺陷。 应该是生物（动物植物杂交于培植）方面的文章吧！  大概是这个意思了。 |
5楼2009-04-13 10:31:24
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zhtt1983(金币+1,VIP+0): 4-20 12:23
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| Transductional crosses were performed with phage 80 as described previously ( 20 ) using as a recipient the parental strain COL (cured from the plasmid pRN3208) and as a donor the newly isolated transposon mutant COL245. Transductants were selected on plates that contained erythromycin at a final concentration of 10 µg/ml. From the cross, 25 transductants were tested for increased levels of methicillin resistance by the 1.0-mg methicillin disk method. Eventually, two transductants were selected for testing by PAP analysis for their antibiotic resistance phenotypes.（Dower and Stahl 1981） |
6楼2009-04-13 10:38:23
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