| 查看: 53 | 回复: 1 | |||
| 当前主题已经存档。 | |||
[交流]
求助文章，深表谢意！
|
|||
| PDA J Pharm Sci Technol. 2005 Sep-Oct;59(5):332-7. |
回复此楼» 猜你喜欢
278求调剂
已经有15人回复
-
284求调剂
已经有15人回复
-
293调剂
已经有16人回复
-
273求调剂
已经有45人回复
-
22408 266求调剂
已经有13人回复
-
266求调剂
已经有15人回复
-
一志愿华东理工085601材料工程303分求调剂
已经有9人回复
-
生物学328分求调剂
已经有4人回复
-
277求调剂
已经有6人回复
-
265求调剂
已经有21人回复
★
jsys(金币+1,VIP+0):感谢参与！ 4-9 15:45
jsys(金币+1,VIP+0):感谢参与！ 4-9 15:45
|
1: PDA J Pharm Sci Technol. 2005 Sep-Oct;59(5):332-7.Links Simple HPLC determination of benzalkonium chloride in ophthalmic formulations containing antazoline and tetrahydrozoline.Rojsitthisak P, Wichitnithad W, Pipitharome O, Sanphanya K, Thanawattanawanich P. Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand. pornchai.r@chula.ac.th A simple and rapid analytical procedure for routine quantification of n-C12H25 and n-C14H29 benzalkonium chloride (C-12 and C-14 BKC) homologs in ophthalmic formulations containing antazoline HCl and tetrahydrozoline HCl by high-performance liquid chromatography was developed and validated. The ophthalmic solution samples can be directly analyzed by reversed-phase on HiQ-Sil C18 column (4.6 mm x 150 mm, i.d., 5-microm particle size) with acetonitrile-sodium acetate buffer (pH 5.0; 0.2 M) (70:30, v/v) as mobile phase. UV Detection was carried out at 262 nm. The method was linear over the selected concentration and ranged from 0.03 to 0.10 mg/ml (r2 = 0.9999) and from 0.01 to 0.05 mg/ml (r2 = 0.9979) for C-12 and C-14 BKC homologs, respectively. The mean percent recoveries were 100.2 and 102.6 and the percent CV values were 1.3 and 3.5 for C-12 and C-14 BKC homologs, respectively. The results demonstrated the good linearity, accuracy, and precision. The method was applied to determine two commercial ophthalmic formulations, and the percent label amounts of total BKC contents were found to be 99.7 and 103.2. |
2楼2009-04-09 15:43:41
