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delia1013木虫 (著名写手)
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[交流]
请问:线粒体DNA编码的蛋白能在大肠杆菌表达吗？
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如题，请高手解答！ I can send you the plasmid pBM5 as described in the Biochemistry paper. It is a pCR-Script vector (from Startagene) containing a PCR fragment covering the mitochondrial cytochrome b intron-less gene, which I used for the construction of most of the cytochrome b mutants. As you know, after mutagenesis, the mutated gene needs to be recombined into the mitochondrial genome. It is not a re-coded gene for nuclear transformation and expression. If you give the name and postal address, I can send a few ul of the plasmid next week if you wish. Brigitte Meunier the gene for cyt b is encoded on mitochondrial DNA and therefore you cannot use expression vectors to express the protein. We do not have such a construct. Brian Robinson. 还是不是很清楚能不能做表达额，高手们帮我看一下~~两个人的回信！！不是学生物的出身，所以很多不明白的~~希望在小木虫得到帮助哈~~~ |
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