| 查看: 73 | 回复: 0 | |||
| 当前主题已经存档。 | |||
delia1013木虫 (著名写手)
|
[交流]
请问:线粒体DNA编码的蛋白能在大肠杆菌表达吗？
|
||
|
如题，请高手解答！ I can send you the plasmid pBM5 as described in the Biochemistry paper. It is a pCR-Script vector (from Startagene) containing a PCR fragment covering the mitochondrial cytochrome b intron-less gene, which I used for the construction of most of the cytochrome b mutants. As you know, after mutagenesis, the mutated gene needs to be recombined into the mitochondrial genome. It is not a re-coded gene for nuclear transformation and expression. If you give the name and postal address, I can send a few ul of the plasmid next week if you wish. Brigitte Meunier the gene for cyt b is encoded on mitochondrial DNA and therefore you cannot use expression vectors to express the protein. We do not have such a construct. Brian Robinson. 还是不是很清楚能不能做表达额，高手们帮我看一下~~两个人的回信！！不是学生物的出身，所以很多不明白的~~希望在小木虫得到帮助哈~~~ |
回复此楼» 猜你喜欢
材料调剂
已经有7人回复
-
化学工程085602 305分求调剂
已经有10人回复
-
289求调剂
已经有15人回复
-
291 求调剂
已经有7人回复
-
274求调剂
已经有14人回复
-
北京林业大学硕导招生广告
已经有3人回复
-
309求调剂
已经有5人回复
-
292求调剂
已经有8人回复
-
求调剂
已经有4人回复
-
一志愿 西北大学 总分282 英语一62 求调剂
已经有3人回复
