| 查看: 71 | 回复: 0 | |||
| 当前主题已经存档。 | |||
delia1013木虫 (著名写手)
|
[交流]
请问:线粒体DNA编码的蛋白能在大肠杆菌表达吗？
|
||
|
如题，请高手解答！ I can send you the plasmid pBM5 as described in the Biochemistry paper. It is a pCR-Script vector (from Startagene) containing a PCR fragment covering the mitochondrial cytochrome b intron-less gene, which I used for the construction of most of the cytochrome b mutants. As you know, after mutagenesis, the mutated gene needs to be recombined into the mitochondrial genome. It is not a re-coded gene for nuclear transformation and expression. If you give the name and postal address, I can send a few ul of the plasmid next week if you wish. Brigitte Meunier the gene for cyt b is encoded on mitochondrial DNA and therefore you cannot use expression vectors to express the protein. We do not have such a construct. Brian Robinson. 还是不是很清楚能不能做表达额，高手们帮我看一下~~两个人的回信！！不是学生物的出身，所以很多不明白的~~希望在小木虫得到帮助哈~~~ |
回复此楼» 猜你喜欢
存款400万可以在学校里躺平吗
已经有15人回复
-
拟解决的关键科学问题还要不要写
已经有6人回复
-
Materials Today Chemistry审稿周期
已经有6人回复
-
基金委咋了？2026年的指南还没有出来？
已经有10人回复
-
基金申报
已经有6人回复
-
推荐一本书
已经有13人回复
-
国自然申请面上模板最新2026版出了吗？
已经有17人回复
-
纳米粒子粒径的测量
已经有8人回复
-
疑惑？
已经有5人回复
-
计算机、0854电子信息（085401-058412）调剂
已经有5人回复