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ÈçÌ⣬Çë¸ßÊÖ½â´ð£¡ I can send you the plasmid pBM5 as described in the Biochemistry paper. It is a pCR-Script vector (from Startagene) containing a PCR fragment covering the mitochondrial cytochrome b intron-less gene, which I used for the construction of most of the cytochrome b mutants. As you know, after mutagenesis, the mutated gene needs to be recombined into the mitochondrial genome. It is not a re-coded gene for nuclear transformation and expression. If you give the name and postal address, I can send a few ul of the plasmid next week if you wish. Brigitte Meunier the gene for cyt b is encoded on mitochondrial DNA and therefore you cannot use expression vectors to express the protein. We do not have such a construct. Brian Robinson. »¹ÊDz»ÊǺÜÇå³þÄܲ»ÄÜ×ö±í´ï¶î£¬¸ßÊÖÃǰïÎÒ¿´Ò»ÏÂ~~Á½¸öÈ˵ĻØÐÅ£¡£¡²»ÊÇѧÉúÎïµÄ³öÉí£¬ËùÒԺܶ಻Ã÷°×µÄ~~Ï£ÍûÔÚСľ³æµÃµ½°ïÖú¹þ~~~ |
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