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| 采用微卫星技术对入侵中国的5个美国白蛾种群进行遗传多样性和遗传分化及影响因素的研究。10对微卫星引物对300个样品DNA进行PCR扩增，并进行聚丙烯酰胺凝胶电泳分析。5个美国白蛾种群的多态位点百分率P=98.36%；多态位点百分率的大小顺序为：沧州种群、烟台种群、秦皇岛种群、丹东种群、石家庄种群；美国白蛾整个群体的遗传分化：34.38 %的变异存在于种群间，65.62%的变异存在于种群内,遗传距离变化范围为0.1386~0.3224。用UPGMA法对Nei′s遗传距离作聚类分析，聚类结果表明：种群的分化程度与分化时间和入侵来源密切相关。影响美国白蛾种群遗传多样性与遗传分化的主要因素是经度、平原比率、年降水量、纬度、入侵时间。美国白蛾种群现有遗传结构的形成与其生活史特性及入侵生态学特性有关。 |
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telomerase(金币+3,VIP+0):酬劳！！！ 4-18 21:54
telomerase(金币+3,VIP+0):酬劳！！！ 4-18 21:54
| The genetic diversity and genetic differentiation and influencing factors of the five White moth invasion species from America to China is studied by Micro-satellite technology 。 10 pairs of microsatellite primers for the 300 samples for PCR amplification of DNA and polyacrylamide gel electrophoresis analysis. Hyphantria cunea five species of the percentage of polymorphic loci P = 98.36%; percentage of polymorphic loci the size of the order: Cangzhou species, species Yantai, Qinhuangdao species, Dandong species, Shijiazhuang species; cunea entire groups of genetic differentiation : 34.38% of variation exists in the inter-species, 65.62% of variation exists in the species, Change the range of genetic distance 0.1386 ~ 0.3224. UPGMA method using Nei's genetic distance for cluster analysis, clustering results show that: the degree of differentiation of species and differentiation of time and invasion is closely related to the source. Hyphantria cunea impact of genetic diversity and species genetic differentiation of the main factors are longitude, Plain ratio of annual precipitation, latitude, invasion time. Hyphantria cunea existing species forming the genetic structure of its life history characteristics and properties of invasion ecology. |
3楼2009-03-19 17:10:45
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telomerase(金币+3,VIP+0):酬劳！！ 4-18 21:54
telomerase(金币+3,VIP+0):酬劳！！ 4-18 21:54
| Micro-satellite technology is used to study the genetic diversity and genetic differentiation and influencing factorsof of the five White moth invasion species from America to China. 10 pairs of microsatellite primers for the 300 samples for PCR amplification of DNA and polyacrylamide gel electrophoresis analysis. Hyphantria cunea five species of the percentage of polymorphic loci P = 98.36%; percentage of polymorphic loci the size of the order: Cangzhou species, species Yantai, Qinhuangdao species, Dandong species, Shijiazhuang species; cunea entire groups of genetic differentiation : 34.38% of variation exists in the inter-species, 65.62% of variation exists in the species, the genetic distance ranged from 0.1386 ~ 0.3224. UPGMA method using Nei's genetic distance for cluster analysis, clustering results show that: the degree of differentiation of species and differentiation of time and invasion is closely related to the source. Hyphantria cunea impact of genetic diversity and species genetic differentiation of the main factors are longitude, Plain ratio of annual precipitation, latitude, invasion time. Hyphantria cunea existing species forming the genetic structure of its life history characteristics and properties of invasion ecology. |
2楼2009-03-19 13:08:07