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aimeiaimei
Òø³æ (ÕýʽдÊÖ)
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- ½ð±Ò: 6.8
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- ³æºÅ: 473224
- ×¢²á: 2007-12-05
- ÐÔ±ð: MM
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3Â¥2009-03-16 19:49:06
sutao1979
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Сľ³æ10Äêgroupleader
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Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10mmols of dNTP into an acid-insoluble material in 30 minutes at 74¡æ. The reaction conditions are: 50mM Tris-HCl, (pH 9.0 at 25 ¡æ ), 50mM NaCl, 10Mm MgCl2, 200uM dATP, dCTP, dGTP and radiolabelled dTTP, and 12.5ug activated calf thymus DNA in a 50ul reaction. ¸ù¾Ýøtaqøµ¥Î»µÄ¶¨Òå¿ÉÒÔ½øÐл»Ë㣬µ«ÊÇ£¬±È½ÏÂé·³ [ Last edited by sutao1979 on 2009-3-15 at 03:06 ] |

2Â¥2009-03-15 03:02:38














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